fear-incubation-using-an-extended-fear-conditioning-protocol-for-rats

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats

Emotional memory has been primarily studied with fear-conditioning paradigms. Fear conditioning is a form of learning through which individuals learn the ...

Fundación Universitaria Konrad Lorenz

Troy University

This paper presents a historical and conceptual analysis of a group of research strategies known as the Single-Case Methods (SCMs). First, we present an overview of the SCMs, their history, and their major proponents. We will argue that the philosophical roots of SCMs can be found in the ideas of authors who recognized the importance of understanding both the generality and individuality of psychological functioning. Second, we will discuss the influence that the natural sciences' attitude toward measurement and experimentation has had on SCMs. Although this influence can be traced back to the early days of experimental psychology, during which incipient forms of SCMs appeared, SCMs reached full development during the subsequent advent of Behavior Analysis (BA). Third, we will show that despite the success of SCMs in BA and other (mainly applied) disciplines, these designs are currently not prominent in psychology. More importantly, they have been neglected as a possible alternative to one of the mainstream approaches in psychology, the Null Hypothesis Significance Testing (NHST), despite serious controversies about the limitations of this prevailing method. Our thesis throughout this section will be that SCMs should be considered as an alternative to NHST because many of the recommendations for improving the use of significance testing (Wilkinson & the TFSI, 1999) are main characteristics of SCMs. The paper finishes with a discussion of a number of the possible reasons why SCMs have been neglected. 

Single-Case Research Methods: History and Suitability for a Psychological Science in Need of Alternatives.

Despite step-down inhibitory avoidance procedures that have been widely implemented in rats and mice to study learning and emotion phenomena, performance of other species in these tasks has received less attention. The case of the Mongolian gerbil is of relevance considering the discrepancies in the parameters of the step-down protocols implemented, especially the wide range of foot-shock intensities (i.e., 0.4-4.0 mA), and the lack of information on long-term performance, extinction effects, and behavioral patterning during these tasks. Experiment 1 aimed to (a) characterize gerbils' acquisition, extinction, and steady-state performance during a multisession (i.e., extended) step-down protocol adapted for implementation in a commercially-available behavioral package (Video Fear Conditioning System-MED Associates Fairfax, VT, USA), and (b) compare gerbils' performance in this task with two shock intensities - 0.5 vs. 1.0 mA-considered in the low-to-mid range. Results indicated that the 1.0 mA protocol produced more reliable and clear evidence of avoidance learning, extinction, and reacquisition in terms of increments in freezing and on-platform time as well as suppression of platform descent. Experiment 2 aimed to (a) assess whether an alternate protocol consisting of a random delivery of foot shocks could replicate the effects of Experiment 1 and (b) characterize gerbils' exploratory behavior during the step-down task (jumping, digging, rearing, and probing). Random shocks did not reproduce the effects observed with the first protocol. The data also indicated that a change from random to response-dependent shocks affects (a) the length of each visit to the platform, but not the frequency of platform descends or freezing time, and (b) the patterns of exploratory behavior, namely, suppression of digging and rearing, as well as increments in probing and jumping. Overall, the study demonstrated the feasibility of the extended step-down protocol for studying steady performance, extinction, and reacquisition of avoidance behavior in gerbils, which could be easily implemented in a commercially available system. The observation that 1.0 mA shocks produced a clear and consistent avoidance behavior suggests that implementation of higher intensities is unnecessary for reproducing aversive-conditioning effects in this species. The observed patterning of freezing, platform descents, and exploratory responses produced by the change from random to periodic shocks may relate to the active defensive system of the gerbil. Of special interest is the probing behavior, which could be interpreted as risk assessment and has not been reported in other rodent species exposed to step-down and similar tasks.

Assessing Mongolian gerbil emotional behavior: effects of two shock intensities and response-independent shocks during an extended inhibitory-avoidance task.

The vast majority of research on aversive control of behavior using animal models employs electric-shock based procedures with avian and mammalian species. Notwithstanding that pragmatic aspects explain this prominence in the field, there is still a need for testing well-established facts about shock-based aversive control using other, perhaps more ecologically valid or biologically relevant, forms of stimulation with other species. Following up on an observation that water disturbances appear to be aversive to Betta splendens (bettas), we developed a preparation for studying free-operant avoidance with this species in which water flows (WFs) replaced electric shocks. Fish changed compartments in a shuttle tank to escape or avoid 10-s WFs, which were delivered with 30-s flow-flow and response-flow intervals. We tested the effect of adding a warning stimulus (curtains of air bubbles) to the last 5 s of the response-flow interval (i.e., signaled avoidance) on the bettas' temporal distribution of crossings and swimming patterns. Escape was the predominant response, which importantly reduced the exposure to the WFs. Avoidance responses rarely exceeded the frequency of escape. The warning stimulus did not produce the expected postponement of responses to the last segment of the response-flow interval. Distinctive swimming patterns emerged, dissipated, and reappeared during baseline and treatment conditions. These findings confirm the aversive function of WFs for bettas and expand the generality of negative reinforcement phenomena using non-shock-based procedures with a less-frequently studied species. Further developments of the WFs paradigm show promise for replicating other aversive control phenomena (e.g., punishment), and analyzing spatiotemporal patterns produced by aversive contingencies.

Aversive control of Betta splendens behavior using water disturbances: Effects of signaled and unsignaled free-operant avoidance and escape contingencies.

Despite the wide implementation of the elevated plus-maze (EPM) test to assess anxiety-related behaviors in rodents, the interpretation of these measures in gerbils has received limited attention. Here, male gerbils were treated with vehicle or diazepam, followed by a 20-min EPM session. EPM data were subjected to minute-by-minute, 5-min bins and factor analyses. During the first 5-min, gerbils avoided the closed arms in favor of the open arms and diazepam increased open-arms entries; furthermore, a single factor (escape behavior) explained all the analyzed measures. Only after 5-min, gerbils reduced open-arms exploration and three independent factors emerged for each subsequent 5-min bin. These findings suggest that EPM data from gerbils should be analyzed in at least two 5-min bins. Measures from the standard 5-min session seem to be related to an escape response from the EPM through the open arms. Once habituated, measures from the second 5-min bin seem to be related to a conflictive situation: keep trying to escape unsuccessfully (due to open-arms height) or seek protection in the closed arms (unsafe places). Diazepam seems to reduce this conflict by mitigating the escape response (Factor 1 - Anxiety) and increasing closed-arms approach (Factor 2) and risk assessment (Factor 3). Unlike mice and rats, a decrease in open-arms exploration and an increase in risk assessment could be interpreted as an anxiolytic-like effect in gerbils.

Diazepam Reduces Escape and Increases Closed-Arms Exploration in Gerbils After 5 min in the Elevated Plus-Maze.

Control of negative emotions (e.g., anger and fear) by political cues perpetuate intractable conflict by mobilizing public support for aggressive actions. Halperin et al. (2013) found that reappraisal - an adaptive form of emotion regulation - decreased negative emotions triggered by anger-inducing information related to the Israeli-Palestinian conflict, and increased support for conciliatory statements. We tested these effects in the context of the conflict between the Colombian government and the Fuerzas Armadas Revolucionarias de Colombia-Ejército del Pueblo (FARC-EP). Reappraisal training reduced negative emotions produced by a presentation that illustrated FARC's violent actions, and increased support for conciliatory statements (with overall moderate effect magnitudes). We also found that negative emotions mediated the effects of reappraisal on the support for aggressive and conciliatory statements. These findings indicate a high degree of generality of the phenomena, especially considering the differences between the Israeli-Palestinian conflict and the Colombian conflict. Our findings also show promise for replicating these effects on other types of intergroup conflicts and guiding effective public policy.

Emotion Regulation and Attitudes Toward Conflict in Colombia: Effects of Reappraisal Training on Negative Emotions and Support for Conciliatory and Aggressive Statements.

The present review assessed the current knowledge regarding caregiver- training effectiveness for human and human-canine dyads. Most canine-related sources (66%; n = 19) were case studies reporting a decrease of learner undesired behavior when using oral instruction (21%; n = 6). Most human-related research used single-case designs (57%; n = 26) reporting an increase in desired learner behavior (22%; n = 10) when caregivers received multi-component training packages (17%, n = 8). The meta-analysis (n = 18) revealed that interventions had a large effect (Hedges' g = 0.88, 95%CI [0.68-1.07]), with packages yielding a slightly larger moderate effect (Hedges' g = 0.76, 95%CI [0.60-0.91]) than oral instruction alone (Hedges' g = 0.74, 95%CI [0.32-1,15]). Although the effectiveness of caregiver training is promising, the results should be interpreted cautiously. Due to the preponderance of case studies within canine literature and the insufficient reporting of data across sources, only few studies could be included in the meta-analysis. Overall, more systematic and comparative research regarding the efficacy of caregivers in behavior change programs across species is needed.

We Don't Train in Vain: A Systematic Review and Meta-Analysis of Human and Canine Caregiver Training.

This paper describes the behavioral data of an experiment in which water flows (WFs) were first used as replacement of the traditional electric shocks to test free-operant avoidance in  (Hurtado-Parrado et al. 2019 https://doi.org/10.1016/j.beproc.2018.10.021). WFs with a duration of 10 s each were delivered with 30-s flow-flow (F-F) and response-flow (R-F) intervals in a custom-made shuttle tank. Fish escaped or avoided the WFs by changing compartments. Crossings during the WFs, interrupted the flows, were automatically scored as escape (Esc), and initiated a new R-F interval. Crossings that occurred during R-F or F-F intervals were scored as avoidance responses and also reset the R-F interval. We compared the effect of adding a warning stimulus - curtains of air bubbles - to the last 5 s of the R-F interval; i.e., signaled versus unsignaled avoidance. A unique development of the WFs procedure, and thus the data here described, is that crossings were further differentiated into subcategories; namely, early avoidance (EA) if a crossing occurred during the first 25 s of the R-F interval; late avoidance (LA) if a crossing occurred during the last 5 s of the R-F interval; and Flow-Flow avoidance (FF) if a crossing occurred anytime during the F-F interval. Here we present the data of six bettas across the different phases of the experiment; namely, baseline (BL - no WFs programmed), signaled avoidance (SA - warning stimulus scheduled), and unsignaled avoidance (UA - no warning stimulus scheduled). The dataset available at the Open Science Framework (OSF) repository (http://doi.org/10.17605/OSF.IO/FMHXD (Hurtado-Parrado et al., 2019)) includes for each fish and per 20-min daily session the total number of crossings; frequency of each type of crossing (Esc, EA, LA, FF); total WF frequency and duration, the total time spent in each compartment, and an index of preference for each compartment based on the proportion of time spent in the tank's compartments.

"Swimming against the current": Behavioral data of  during an escape and avoidance task with water flows as the aversive stimulus.

The aim of this study was to investigate the feasibility and effectiveness of procedures successfully used in human related applied behaviour analysis practices to the field of clinical animal behaviour. Experiment 1 involved functional analyses to identify the reinforcement contingencies maintaining jumping up behaviour in five dogs. Experiment 2 comprised teaching dog owners a noncontingent reinforcement intervention (i.e., time-based reinforcement) via behavioural skills training. Single-case experimental methods were implemented in both experiments. The results of Experiment 1 showed that access to a tangible (dogs D01, D02, D03, and D04) and owner attention (dog D05) were reliably maintaining the jumping up behaviour. Experiment 2 demonstrated that noncontingent reinforcement effectively reduced jumping in three out of four dogs (Tau -0.59, CI 90% [-1-0.15],  = 0.026, Tau -1, CI 90% [-1--0.55],  = 0.0003, and Tau -0.32, CI 90% [-0.76-0.11],  = 0.22 for dyads D01, D02, and D05, respectively), and that behavioural skills training was successful in teaching owners to perform a dog training intervention with high fidelity. Although the results are promising, more canine-related research into functional analysis and noncontingent reinforcement, as well as implementation of behavioural skills training with animal caregivers, is needed.

Article	Year
Single-Case Research Methods: History and Suitability for a Psychological Science in Need of Alternatives. Integrative psychological & behavioral science\| PubMed ID: 25876996	2015
Assessing Mongolian gerbil emotional behavior: effects of two shock intensities and response-independent shocks during an extended inhibitory-avoidance task. PeerJ\| PubMed ID: 29152417	2017
Aversive control of Betta splendens behavior using water disturbances: Effects of signaled and unsignaled free-operant avoidance and escape contingencies. Behavioural processes\| PubMed ID: 30391658	2019
Diazepam Reduces Escape and Increases Closed-Arms Exploration in Gerbils After 5 min in the Elevated Plus-Maze. Frontiers in psychology\| PubMed ID: 31001181	2019
Emotion Regulation and Attitudes Toward Conflict in Colombia: Effects of Reappraisal Training on Negative Emotions and Support for Conciliatory and Aggressive Statements. Frontiers in psychology\| PubMed ID: 31068877	2019
We Don't Train in Vain: A Systematic Review and Meta-Analysis of Human and Canine Caregiver Training. Journal of applied animal welfare science : JAAWS\| PubMed ID: 31394050	2020
"Swimming against the current": Behavioral data of during an escape and avoidance task with water flows as the aversive stimulus. Data in brief\| PubMed ID: 31406902	2019
Using Principles from Applied Behaviour Analysis to Address an Undesired Behaviour: Functional Analysis and Treatment of Jumping Up in Companion Dogs. Animals : an open access journal from MDPI\| PubMed ID: 31817670	2019
Editorial: Research on Emotion and Learning: Contributions from Latin America. Frontiers in psychology\| PubMed ID: 32318003	2020

Camilo Hurtado-Parrado

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