Blaine A. Pfeifer

Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli.

A specific role of the Saccharopolyspora erythraea thioesterase II gene in the function of modular polyketide synthases.

Precursor-Directed polyketide biosynthesis in Escherichia coli.

Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host.

Formulation and surface modification of poly(ester-anhydride) micro- and nanospheres.

Poly(ester-anhydride):poly(beta-amino ester) micro- and nanospheres: DNA encapsulation and cellular transfection.

Improving heterologous polyketide production in Escherichia coli by overexpression of an S-adenosylmethionine synthetase gene.

6-deoxyerythronolide B production through chromosomal localization of the deoxyerythronolide B synthase genes in E. coli.

Polyketide analysis using mass spectrometry, evaporative light scattering, and charged aerosol detector systems.

Bacterial hosts for natural product production.

A comparison between polymeric microsphere and bacterial vectors for macrophage P388D1 gene delivery.

Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli.

A high-throughput comparison of recombinant gene expression parameters for E. coli-mediated gene transfer to P388D1 macrophage cells.

Efficient experimental design and micro-scale medium enhancement of 6-deoxyerythronolide B production through Escherichia coli.

Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli.

Computational analysis of phenotypic space in heterologous polyketide biosynthesis--applications to Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae.

Metabolic flux analysis and pharmaceutical production.

Utilizing elementary mode analysis, pathway thermodynamics, and a genetic algorithm for metabolic flux determination and optimal metabolic network design.

Methods and options for the heterologous production of complex natural products.

Complete biosynthesis of erythromycin A and designed analogs using E. coli as a heterologous host.

Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in Escherichia coli.

Multi-factorial engineering of heterologous polyketide production in Escherichia coli reveals complex pathway interactions.

Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an Escherichia coli two-phase bioprocess.

Analysis of heterologous taxadiene production in K- and B-derived Escherichia coli.

Improved E. coli erythromycin A production through the application of metabolic and bioprocess engineering.

Computational identification of gene over-expression targets for metabolic engineering of taxadiene production.

Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds.

Downstream reactions and engineering in the microbially reconstituted pathway for Taxol.