Eckhard Wolf

Changes of higher order chromatin arrangements during major genome activation in bovine preimplantation embryos.

Systemic first-line phenotyping.

Maternal inexperience as a risk factor of innate fear and PTSD-like symptoms in mice.

The endometrium responds differently to cloned versus fertilized embryos.

Scrapie-infected transgenic mice expressing a laminin receptor decoy mutant reveal a prolonged incubation time associated with low levels of PrPres.

The C-terminal cytoplasmic domain of human proEGF is a negative modulator of body and organ weights in transgenic mice.

Distribution and expression of porcine endogenous retroviruses in multi-transgenic pigs generated for xenotransplantation.

A new Fgf10 mutation in the mouse leads to atrophy of the harderian gland and slit-eye phenotype in heterozygotes: a novel model for dry-eye disease?

A humanized version of Foxp2 affects cortico-basal ganglia circuits in mice.

Chronic growth hormone excess is associated with increased aldosterone: a study in patients with acromegaly and in growth hormone transgenic mice.

Evidence for estrogen-dependent uterine serpin (SERPINA14) expression during estrus in the bovine endometrial glandular epithelium and lumen.

N-ethyl-N-nitrosourea mutagenesis produced a small number of mice with altered plasma electrolyte levels.

Reprogramming of active and repressive histone modifications following nuclear transfer with rabbit mesenchymal stem cells and adult fibroblasts.

Dll1 haploinsufficiency in adult mice leads to a complex phenotype affecting metabolic and immunological processes.

Clinical chemistry of congenic mice with quantitative trait loci for predicted responses to Trypanosoma congolense infection.

Specific transgene expression in mouse pancreatic beta-cells under the control of the porcine insulin promoter.

Novel missense mutation of uromodulin in mice causes renal dysfunction with alterations in urea handling, energy, and bone metabolism.

Epigen transgenic mice develop enlarged sebaceous glands.

Germ-line transmission of lentiviral PGK-EGFP integrants in transgenic cattle: new perspectives for experimental embryology.

EGFR ligands exert diverging effects on male reproductive organs.

Genetic modification of pigs as organ donors for xenotransplantation.

Microphthalmia, parkinsonism, and enhanced nociception in Pitx3 ( 416insG ) mice.

Betacellulin protects from pancreatitis by activating stress-activated protein kinase.

Transgenic pigs for xenotransplantation: selection of promoter sequences for reliable transgene expression.

Escherichia coli infection induces distinct local and systemic transcriptome responses in the mammary gland.

Glucose intolerance and reduced proliferation of pancreatic beta-cells in transgenic pigs with impaired glucose-dependent insulinotropic polypeptide function.

Loss of the actin remodeler Eps8 causes intestinal defects and improved metabolic status in mice.

Mutation of the Na(+)-K(+)-2Cl(-) cotransporter NKCC2 in mice is associated with severe polyuria and a urea-selective concentrating defect without hyperreninemia.

Transgenic pigs as models for translational biomedical research.

Differential endometrial gene expression in pregnant and nonpregnant sows.

Genomic integration of adenoviral gene transfer vectors following transduction of fertilized mouse oocytes.

Quantification of leukocyte genomic 5-methylcytosine levels reveals epigenetic plasticity in healthy adult cloned cattle.

Mouse phenotyping.

FISH on 3D preserved bovine and murine preimplantation embryos.

Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium.

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy.

Utilization of a mutagenesis screen to generate mouse models of hyperaldosteronism.

A key role for E-cadherin in intestinal homeostasis and Paneth cell maturation.

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species.

A novel N-ethyl-N-nitrosourea-induced mutation in phospholipase Cγ2 causes inflammatory arthritis, metabolic defects, and male infertility in vitro in a murine model.

Decreased incidence of papillomas in mice with impaired EGFR function during multi-stage skin carcinogenesis.

Increase of essential amino acids in the bovine uterine lumen during preimplantation development.

Missing-in-metastasis MIM/MTSS1 promotes actin assembly at intercellular junctions and is required for integrity of kidney epithelia.

Requirement of the RNA-editing enzyme ADAR2 for normal physiology in mice.

Generation of N-ethyl-N-nitrosourea-induced mouse mutants with deviations in hematological parameters.

Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells.

Reduced amino acids in the bovine uterine lumen of cloned versus in vitro fertilized pregnancies prior to implantation.

Cell arrest and cell death in mammalian preimplantation development: lessons from the bovine model.

Postnatal development of numbers and mean sizes of pancreatic islets and beta-cells in healthy mice and GIPR(dn) transgenic diabetic mice.

In vivo evidence for epidermal growth factor receptor (EGFR)-mediated release of prolactin from the pituitary gland.

A transgenic mouse line expressing cre recombinase in pancreatic β-cells.

Lack of Pur-alpha alters postnatal brain development and causes megalencephaly.

Gene expression profiling of bovine peripartal placentomes: detection of molecular pathways potentially involved in the release of foetal membranes.

Comparison of the effects of early pregnancy with human interferon, alpha 2 (IFNA2), on gene expression in bovine endometrium.

Normal epidermal growth factor receptor signaling is dispensable for bone anabolic effects of parathyroid hormone.

First inducible transgene expression in porcine large animal models.

Clinical chemistry of human FcRn transgenic mice.

Negative feedback mechanisms surpass the effect of intrinsic EGFR activation during skin chemical carcinogenesis.

Human TNF-related apoptosis-inducing ligand-expressing dendritic cells from transgenic pigs attenuate human xenogeneic T cell responses.

Neurobeachin, a regulator of synaptic protein targeting, is associated with body fat mass and feeding behavior in mice and body-mass index in humans.

Changing metabolic signatures of amino acids and lipids during the prediabetic period in a pig model with impaired incretin function and reduced β-cell mass.

Xenografted islet cell clusters from INSLEA29Y transgenic pigs rescue diabetes and prevent immune rejection in humanized mice.

New mouse models for metabolic bone diseases generated by genome-wide ENU mutagenesis.

A new mouse model for studying EGFR-dependent gastric polyps.

Cytomegalovirus replicon-based regulation of gene expression in vitro and in vivo.

Large-scale phenotyping of an accurate genetic mouse model of JNCL identifies novel early pathology outside the central nervous system.

Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology.

Does enamelin have pleiotropic effects on organs other than the teeth? Lessons from a phenotyping screen of two enamelin-mutant mouse lines.

Induction of oxazolone-mediated features of atopic dermatitis in NOD-scid IL2Rγ(null) mice engrafted with human peripheral blood mononuclear cells.

A porcine model of familial adenomatous polyposis.

Transcriptome analyses of bovine, porcine and equine endometrium during the pre-implantation phase.

Innovations in phenotyping of mouse models in the German Mouse Clinic.

In-vivo biodegradation of extruded lipid implants in rabbits.

The hepatic phosphatidylcholine transporter ABCB4 as modulator of glucose homeostasis.

Inactivation and inducible oncogenic mutation of p53 in gene targeted pigs.

Truncation of MIMT1 gene in the PEG3 domain leads to major changes in placental gene expression and stillbirth in cattle.

Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig.

Genetic deletion of the EGFR ligand epigen does not affect mouse embryonic development and tissue homeostasis.

Completion of the swine genome will simplify the production of swine as a large animal biomedical model.

The endocytic adaptor Eps15 controls marginal zone B cell numbers.

Hosting the preimplantation embryo: potentials and limitations of different approaches for analysing embryo-endometrium interactions in cattle.

Permanent neonatal diabetes in INS(C94Y) transgenic pigs.

NOD-scid IL2R γnull mice engrafted with human peripheral blood mononuclear cells as a model to test therapeutics targeting human signaling pathways.

Immune aspects of embryo-maternal cross-talk in the bovine uterus.

SMC6 is an essential gene in mice, but a hypomorphic mutant in the ATPase domain has a mild phenotype with a range of subtle abnormalities.

Modeling hepatic osteodystrophy in Abcb4 deficient mice.

Tissue-specific and minor inter-individual variation in imprinting of IGF2R is a common feature of Bos taurus Concepti and not correlated with fetal weight.

Mouse nuclear myosin I knock-out shows interchangeability and redundancy of myosin isoforms in the cell nucleus.

iTRAQ proteome analysis reflects a progressed differentiation state of epiblast derived versus inner cell mass derived murine embryonic stem cells.

Cytoplasmic mislocalization of POU3F4 due to novel mutations leads to deafness in humans and mice.

High mobility group N proteins modulate the fidelity of the cellular transcriptional profile in a tissue- and variant-specific manner.

Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set.

Type of uromodulin mutation and allelic status influence onset and severity of uromodulin-associated kidney disease in mice.

Dystrophin-deficient pigs provide new insights into the hierarchy of physiological derangements of dystrophic muscle.

An ENU mutagenesis-derived mouse model with a dominant Jak1 mutation resembling phenotypes of systemic autoimmune disease.

Rapamycin extends murine lifespan but has limited effects on aging.

Increased activation of the epidermal growth factor receptor in transgenic mice overexpressing epigen causes peripheral neuropathy.

Activation of the lectin pathway of complement in pig-to-human xenotransplantation models.

Expression pattern of STAT5A gene during early bovine embryogenesis.

Genetically engineered pig models for diabetes research.

Ligand-independent epidermal growth factor receptor hyperactivation increases sebaceous gland size and sebum secretion in mice.

Regulatory sequences of the porcine THBD gene facilitate endothelial-specific expression of bioactive human thrombomodulin in single- and multitransgenic pigs.

Standardized, systemic phenotypic analysis of Umod(C93F) and Umod(A227T) mutant mice.

A broad phenotypic screen identifies novel phenotypes driven by a single mutant allele in Huntington's disease CAG knock-in mice.

Protein O-mannosylation is crucial for E-cadherin-mediated cell adhesion.

Phenotypic comparison of common mouse strains developing high-fat diet-induced hepatosteatosis.

Comparative and retrospective molecular analysis of Parapoxvirus (PPV) isolates.

The ABC of BTC: structural properties and biological roles of betacellulin.

Mitochondrial dysfunction and decrease in body weight of a transgenic knock-in mouse model for TDP-43.

No amelioration of uromodulin maturation and trafficking defect by sodium 4-phenylbutyrate in vivo: studies in mouse models of uromodulin-associated kidney disease.

Fine mapping of genome activation in bovine embryos by RNA sequencing.

Homologous recombination contributes to the repair of zinc-finger-nuclease induced double strand breaks in pig primary cells and facilitates recombination with exogenous DNA.

Complement dependent early immunological responses during ex vivo xenoperfusion of hCD46/HLA-E double transgenic pig forelimbs with human blood.

Abnormal brain iron metabolism in Irp2 deficient mice is associated with mild neurological and behavioral impairments.

Genome activation in bovine embryos: review of the literature and new insights from RNA sequencing experiments.

Uniformity of nucleosome preservation pattern in Mammalian sperm and its connection to repetitive DNA elements.

Dual fluorescent reporter pig for Cre recombination: transgene placement at the ROSA26 locus.

DRO1 inactivation drives colorectal carcinogenesis in ApcMin/+ mice.

Metformin supports the antidiabetic effect of a sodium glucose cotransporter 2 inhibitor by suppressing endogenous glucose production in diabetic mice.

Standardized, systemic phenotypic analysis of Slc12a1I299F mutant mice.

Stage-specific proteome signatures in early bovine embryo development.

Pleiotropic functions for transcription factor zscan10.

Proteome analysis of early lineage specification in bovine embryos.

Nuclear transfer and transgenesis in the pig.

Calcitonin controls bone formation by inhibiting the release of sphingosine 1-phosphate from osteoclasts.

Uterine responses to the preattachment embryo in domestic ungulates: recognition of pregnancy and preparation for implantation.

Uromodulin retention in thick ascending limb of Henle's loop affects SCD1 in neighboring proximal tubule: renal transcriptome studies in mouse models of uromodulin-associated kidney disease.

Reprogramming of fibroblast nuclei in cloned bovine embryos involves major structural remodeling with both striking similarities and differences to nuclear phenotypes of in vitro fertilized embryos.

Positional changes of a pluripotency marker gene during structural reorganization of fibroblast nuclei in cloned early bovine embryos.

Screen for alterations of iron related parameters in N-ethyl-N-nitrosourea-treated mice identified mutant lines with increased plasma ferritin levels.

Efavirenz Causes Oxidative Stress, Endoplasmic Reticulum Stress, and Autophagy in Endothelial Cells.

Commentary on "Meta-analysis of the independent and cumulative effects of multiple genetic modifications on pig lung xenograft performance during ex vivo perfusion with human blood" (by Harris et al.): tailoring donor pigs for xenotransplantation-how to find the right combination of genetic modifications?

Viable pigs with a conditionally-activated oncogenic KRAS mutation.

Glucose tolerance tests for systematic screening of glucose homeostasis in mice.

Pig-to-baboon heterotopic heart transplantation--exploratory preliminary experience with pigs transgenic for human thrombomodulin and comparison of three costimulation blockade-based regimens.

Inactivation of Itf2 promotes intestinal tumorigenesis in Apc(Min/+) mice.

Effects of the glucagon-like peptide-1 receptor agonist liraglutide in juvenile transgenic pigs modeling a pre-diabetic condition.

Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications.

Virus safety of islet cell transplantation from transgenic pigs to marmosets.

Transgenic Expression of Human CD46 on Porcine Endothelium: Effect on Coagulation and Fibrinolytic Cascades During Ex Vivo Human-to-Pig Limb Xenoperfusions.

Dysregulated IGFBP5 expression causes axon degeneration and motoneuron loss in diabetic neuropathy.

Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum.

Blood Collection from Mice and Hematological Analyses on Mouse Blood.

Functional compensation among HMGN variants modulates the DNase I hypersensitive sites at enhancers.

Analysis of mammalian gene function through broad-based phenotypic screens across a consortium of mouse clinics.

Xenotransplantation of Cells, Tissues, Organs and the German Research Foundation Transregio Collaborative Research Centre 127.

eIF6 coordinates insulin sensitivity and lipid metabolism by coupling translation to transcription.

Phytohemagglutinin facilitates the aggregation of blastomere pairs from Day 5 donor embryos with Day 4 host embryos for chimeric bovine embryo multiplication.

Metamizol Relieves Pain Without Interfering With Cerulein-Induced Acute Pancreatitis in Mice.

Incretin actions and consequences of incretin-based therapies: lessons from complementary animal models.

Pigs pave a way to de novo formation of functional human kidneys.

Engraftment and reversal of diabetes after intramuscular transplantation of neonatal porcine islet-like clusters.

Dissociation of somatic growth, time of sexual maturity, and life expectancy by overexpression of an RGD-deficient IGFBP-2 variant in female transgenic mice.

Tailored Pig Models for Preclinical Efficacy and Safety Testing of Targeted Therapies.

MFAP4 Promotes Vascular Smooth Muscle Migration, Proliferation and Accelerates Neointima Formation.

Computed Tomography (CT) Scanning Facilitates Early Identification of Neonatal Cystic Fibrosis Piglets.

Pre-clinical heterotopic intrathoracic heart xenotransplantation: a possibly useful clinical technique.

3D structured illumination microscopy of mammalian embryos and spermatozoa.

Stage-dependent remodeling of the nuclear envelope and lamina during rabbit early embryonic development.

Developmental endothelial locus-1 modulates platelet-monocyte interactions and instant blood-mediated inflammatory reaction in islet transplantation.

Pillars Article: CCR7 Coordinates the Primary Immune Response by Establishing Functional Microenvironments in Secondary Lymphoid Organs. Cell. 1999. 99: 23-33.

Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice.

Inhibition of complement component C5 prevents clotting in an ex vivo model of xenogeneic activation of coagulation.

Exome sequencing identifies a nonsense mutation in Fam46a associated with bone abnormalities in a new mouse model for skeletal dysplasia.

Mildly compromised tetrahydrobiopterin cofactor biosynthesis due to Pts variants leads to unusual body fat distribution and abdominal obesity in mice.

Tissue Sampling Guides for Porcine Biomedical Models.

First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes--Chapter 2b: genetically modified source pigs.

Growth hormone (GH)-transgenic insulin-like growth factor 1 (IGF1)-deficient mice allow dissociation of excess GH and IGF1 effects on glomerular and tubular growth.

Generation and Standardized, Systemic Phenotypic Analysis of Pou3f3L423P Mutant Mice.

Chimeric 2C10R4 anti-CD40 antibody therapy is critical for long-term survival of GTKO.hCD46.hTBM pig-to-primate cardiac xenograft.

Transgenic Expression of Human Thrombomodulin Inhibits HMGB1-Induced Porcine Aortic Endothelial Cell Activation.

Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs.

Comparative aspects of rodent and nonrodent animal models for mechanistic and translational diabetes research.

Bezafibrate Improves Insulin Sensitivity and Metabolic Flexibility in STZ-Induced Diabetic Mice.

Efficient production of multi-modified pigs for xenotransplantation by 'combineering', gene stacking and gene editing.

Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle.

Clinical Chemistry Reference Intervals for C57BL/6J, C57BL/6N, and C3HeB/FeJ Mice (Mus musculus).

Progress in Clinical Encapsulated Islet Xenotransplantation.

A mouse model for ulcerative colitis based on NOD-scid IL2R γnull mice reconstituted with peripheral blood mononuclear cells from affected individuals.

Progressive muscle proteome changes in a clinically relevant pig model of Duchenne muscular dystrophy.

Loss of DRO1/CCDC80 results in obesity and promotes adipocyte differentiation.

Viable Ednra (Y129F) mice feature human mandibulofacial dysostosis with alopecia (MFDA) syndrome due to the homologue mutation.

The First Scube3 Mutant Mouse Line with Pleiotropic Phenotypic Alterations.

Serum Response Factor (SRF) Ablation Interferes with Acute Stress-Associated Immediate and Long-Term Coping Mechanisms.

Direct introduction of gene constructs into the pronucleus-like structure of cloned embryos: a new strategy for the generation of genetically modified pigs.

Diabetes Mellitus-Induced Microvascular Destabilization in the Myocardium.

Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease - UMOD (ADTKD-UMOD).

Modification of the fatty acid composition of an obesogenic diet improves the maternal and placental metabolic environment in obese pregnant mice.

Bezafibrate ameliorates diabetes via reduced steatosis and improved hepatic insulin sensitivity in diabetic TallyHo mice.

INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters.

Vessel network architecture of adult human islets promotes distinct cell-cell interactions in situ and is altered after transplantation.

Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes.

Retinopathy with central oedema in an INS (C94Y) transgenic pig model of long-term diabetes.

Abstracts of the 52nd Workshop for Pediatric Research : Frankfurt, Germany. 27-28 October 2016.

Extensive phenotypic characterization of a new transgenic mouse reveals pleiotropic perturbations in physiology due to mesenchymal hGH minigene expression.

Meis1: effects on motor phenotypes and the sensorimotor system in mice.

Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood.

The Munich MIDY Pig Biobank - A unique resource for studying organ crosstalk in diabetes.

Every-other-day feeding extends lifespan but fails to delay many symptoms of aging in mice.

Standardized, systemic phenotypic analysis reveals kidney dysfunction as main alteration of Kctd1 (I27N) mutant mice.