Romain Fontaine

Department of Basic Sciences and Aquatic Medicine,
Faculty of Veterinary Medicine,
Physiology Unit,
Department of Preclinical Sciences and Pathology,
Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine,
Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine,
Physiology Unit, Faculty of Veterinary Medicine

Norwegian University of Life Sciences

BIOGRAPHY

I obtained a Ph.D. in neurosciences in 2015 for my work on the dopaminergic inhibitory system controlling the reproductive function in zebrafish. Currently, I work on the reproductive physiology in fish, as a neuroendocrinologist in the group of Prof. Finn-Arne Weltzien. I study and characterize the gonadotopes cells in the pituitary looking at the ontogeny, plasticity and cell communication as well as the effect of some environmental factors on their activity. For this purpose, I mostly use fluorescent in situ hybridization, immunofluorescence, ELISA and confocal imaging techniques, and the Atlantic salmon and the medaka as model animals.

PUBLICATIONS

Medaka follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression levels.

General and comparative endocrinology 02, 2019 | Pubmed ID: 30576646

Plasticity of Lh cells caused by cell proliferation and recruitment of existing cells.

The Journal of endocrinology 02, 2019 | Pubmed ID: 30594119

Data on Western blot and ELISA analysis of medaka () follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with .

Data in brief Feb, 2019 | Pubmed ID: 30740493

Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis.

Scientific reports Mar, 2019 | Pubmed ID: 30872608

Melatonin receptors in Atlantic salmon stimulate cAMP levels in heterologous cell lines and show season-dependent daily variations in pituitary expression levels.

Journal of pineal research Jun, 2019 | Pubmed ID: 31169933

Establishment of specific enzyme-linked immunosorbent assay (ELISA) for measuring Fsh and Lh levels in medaka (), using recombinant gonadotropins.

MethodsX , 2019 | Pubmed ID: 31293904

Cold seawater induces early sexual developmental stages in the BPG axis of European eel males.

BMC genomics Jul, 2019 | Pubmed ID: 31331264

Gnrh receptor gnrhr2bbα is expressed exclusively in lhb-expressing cells in Atlantic salmon male parr.

General and comparative endocrinology 01, 2020 | Pubmed ID: 31580881

Gnrh1-Induced Responses Are Indirect in Female Medaka Fsh Cells, Generated Through Cellular Networks.

Endocrinology 12, 2019 | Pubmed ID: 31621882

Gonadotrope plasticity at cellular, population and structural levels: A comparison between fishes and mammals.

General and comparative endocrinology 02, 2020 | Pubmed ID: 31794734

Plasticity in medaka gonadotropes via cell proliferation and phenotypic conversion.

The Journal of endocrinology 04, 2020 | Pubmed ID: 31977313

Direct and Indirect Effects of Sex Steroids on Gonadotrope Cell Plasticity in the Teleost Fish Pituitary.

Frontiers in endocrinology , 2020 | Pubmed ID: 33365013

Effects of Melatonin on Anterior Pituitary Plasticity: A Comparison Between Mammals and Teleosts.

Frontiers in endocrinology , 2020 | Pubmed ID: 33505357

Long extensions with varicosity-like structures in gonadotrope Lh cells facilitate clustering in medaka pituitary culture.

PloS one , 2021 | Pubmed ID: 33507913

Editorial: Plasticity in the Vertebrate Pituitary, Including Regulatory Mechanisms.

Frontiers in endocrinology , 2021 | Pubmed ID: 34084154

3D Atlas of the Pituitary Gland of the Model Fish Medaka ().

Frontiers in endocrinology , 2021 | Pubmed ID: 34497587

Characterization of hormone-producing cell types in the teleost pituitary gland using single-cell RNA-seq.

Scientific data 10, 2021 | Pubmed ID: 34711832

Pituitary multi-hormone cells in mammals and fish: history, origin, and roles.

Frontiers in neuroendocrinology Jul, 2022 | Pubmed ID: 35870647

INSTITUTIONS

Norwegian University of Life Sciences

JOVE ARTICLES

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Romain Fontaine^*,1,

Kjetil Hodne^*,1,

Finn-Arne Weltzien¹

¹Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

Neuroscience

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Eirill Ager-Wick¹,

Kjetil Hodne¹,

Romain Fontaine¹,

Kristine von Krogh¹,

Trude M. Haug²,

Finn-Arne Weltzien¹

¹Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences,

²Department of Oral Biology, Faculty of Dentistry, University of Oslo

Neuroscience

Labeling of Blood Vessels in the Teleost Brain and Pituitary Using Cardiac Perfusion with a DiI-fixative

Romain Fontaine¹,

Finn-Arne Weltzien¹

¹Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

Bioengineering

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

Lauren Closs¹,

Amin Sayyari²,

Romain Fontaine¹

¹Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences,

²Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

Biology

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Muhammad Rahmad Royan¹,

Shinji Kanda²,

Daichi Kayo³,

Weiyi Song⁴,

Wei Ge⁴,

Finn-Arne Weltzien¹,

Romain Fontaine¹

¹Physiology Unit, Faculty of Veterinary Medicine, Norwegian University of Life Sciences,

²Laboratory of Physiology, Atmosphere and Ocean Research Institute, The University of Tokyo,

³Department of Biological Sciences, Graduate School of Science, The University of Tokyo,

⁴Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau

Biology

Privacy

Policies

Recommend to library

JoVE NEWSLETTERS

Research

JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive

Education

JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Faculty Site

Authors

Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit

Librarians

Overview
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
FAQ

ABOUT JoVE

Overview
Leadership
Blog
JoVE Help Center