Sean M. Hemmingsen

Extensive profiling of a complex microbial community by high-throughput sequencing.

Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe.

Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris.

Enhancing Escherichia coli electrotransformation competency by invoking physiological adaptations to stress and modifying membrane integrity.

Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp.

cpnDB: a chaperonin sequence database.

Modeling the structure of the type I peritrophic matrix: characterization of a Mamestra configurata intestinal mucin and a novel peritrophin containing 19 chitin binding domains.

Comparison of ileum microflora of pigs fed corn-, wheat-, or barley-based diets by chaperonin-60 sequencing and quantitative PCR.

Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti.

Enumeration of specific bacterial populations in complex intestinal communities using quantitative PCR based on the chaperonin-60 target.

Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in Schizosaccharomyces pombe.

Dissecting the domain structure of Cdc4p, a myosin essential light chain involved in Schizosaccharomyces pombe cytokinesis.

Characterization of vaginal microflora of healthy, nonpregnant women by chaperonin-60 sequence-based methods.

Identification of pathogenic Helicobacter species by chaperonin-60 differentiation on plastic DNA arrays.

Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database.

Improved template representation in cpn60 polymerase chain reaction (PCR) product libraries generated from complex templates by application of a specific mixture of PCR primers.

Characterization of intestinal microbiota and response to dietary virginiamycin supplementation in the broiler chicken.

Biochemical and taxonomic characterization of bacteria associated with the crucifer root maggot (Delia radicum).

Molecular characterization of microbial communities in Canadian pulp and paper activated sludge and quantification of a novel Thiothrix eikelboomii-like bulking filament.

Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening.

A new fluorescent quantitative PCR-based in vitro neutralization assay for white spot syndrome virus.

Essential role for Schizosaccharomyces pombe pik1 in septation.

Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species.

Pyrosequencing of chaperonin-60 (cpn60) amplicons as a means of determining microbial community composition.