1Molecular and Integrative Physiology Department, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor
* These authors contributed equally
Summary
The food source for Caenorhabditis elegans in the lab is live Escherichia coli. Since bacteria are metabolically active, they present a confounding variable in metabolic and drug studies in C. elegans. A detailed protocol to metabolically inactivate bacteria using paraformaldehyde is described here.
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