University Medical Center Mainz, Johannes Gutenberg-University Mainz

1 ARTICLES PUBLISHED IN JoVE

Medicine

Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital Microscopy

Zhenling Gao¹, Olga Dremova¹, Cornelia Karwot¹, Christoph Reinhardt^*1,2, Klytaimnistra Kiouptsi^*1,2

¹Center for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Johannes Gutenberg-University Mainz, ²German Center for Cardiovascular Research (DZHK), Partner Site Rhine-Main

This protocol presents a method for inducing mesenteric ischemia-reperfusion injury and visualizing neutrophil extracellular traps (NETs) in mesenteric venules using intravital microscopy. Blood flow in the superior mesenteric artery was restricted for 1 h, followed by reperfusion, allowing direct quantification of leukocyte adhesion and NETosis.