City University of New York

4 ARTICLES PUBLISHED IN JoVE

Behavior

Lexical Decision Task for Studying Written Word Recognition in Adults with and without Dementia or Mild Cognitive Impairment

Alexandre Nikolaev¹, Eve Higby^2,3, JungMoon Hyun⁴, Sameer Ashaie^5,6

¹Helsinki Collegium for Advanced Studies, University of Helsinki, ²University of California, Riverside, ³California State University, East Bay, ⁴Hunter College, City University of New York, ⁵Feinberg School of Medicine, Northwestern University, ⁶Shirley Ryan AbilityLab

This article describes how to implement a simple lexical decision experiment to assess written word recognition in neurologically healthy participants and in individuals with dementia and cognitive decline. We also provide a detailed description of reaction time analysis using principal components analysis (PCA) and mixed-effects modeling.

Genetics

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Ning Zhang^1,2, Shundi Shi², Barney Yoo³, Xiaohong Yuan¹, Wenjia Li⁴, Shenglong Zhang¹

¹Department of Biological and Chemical Sciences, New York Institute of Technology, ²Department of Chemical Engineering, Columbia University, ³Department of Chemistry, Hunter College, City University of New York, ⁴Department of Computer Science, New York Institute of Technology

Here, we describe a detailed protocol for an LC-MS-based sequencing method that can be used as a direct method to sequence short RNA (<35 nt per run) without a cDNA intermediate, and as a general method to sequence different nucleotide modifications in a single study at single-base precision.

Environment

Simulating Impacts of Ice Storms on Forest Ecosystems

John L. Campbell¹, Lindsey E. Rustad¹, Charles T. Driscoll², Ian Halm³, Timothy J. Fahey⁴, Habibollah Fakhraei⁵, Peter M. Groffman^6,7, Gary J. Hawley⁸, Wendy Leuenberger⁹, Paul G. Schaberg¹⁰

¹Northern Research Station, U.S. Forest Service, Durham, NH, ²Department of Civil and Environmental Engineering, Syracuse University, ³Northern Research Station, U.S. Forest Service, North Woodstock, NH, ⁴Department of Natural Resources, Cornell University, ⁵Department of Civil and Environmental Engineering, Southern Illinois University, ⁶Advanced Science Research Center at the Graduate Center, City University of New York, ⁷Cary Institute of Ecosystem Studies, ⁸Rubenstein School of Environment and Natural Resources, University of Vermont, ⁹Department of Forestry and Natural Resources, University of Kentucky, ¹⁰Northern Research Station, U.S. Forest Service, Burlington, VT

Ice storms are important weather events that are challenging to study because of difficulties in predicting their occurrence. Here, we describe a novel method for simulating ice storms that involves spraying water over a forest canopy during sub-freezing conditions.

Experimental Entomology in the Age of Video

Daniel A. Friedman¹, Judith R. Wexler², Sebastian Alvarado³

¹Department of Entomology & Nematology, University of California, ²Department of Ecology, Evolution, and Behavior, The Hebrew University in Jerusalem, ³Department of Biology, Queens College, City University of New York

Experimental Entomology in the Age of Video