Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB PathogenesisPriyadarshini Sanyal 1,2, Vinay Kumar Nandicoori 1,2,3
1CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), 2Academy of Scientific and Innovative Research (AcSIR), 3National Institute of Immunology
Here, we demonstrate the preparation of Mtb culture filtrate (CF), which is the bacterial growth media that consists of proteins secreted by the bacteria. Mtb secretes proteins to modulate host signaling and immune response, which are crucial for pathogenesis. The CFs prepared are analyzed by mass spectrometry to identify all the secretory factors.
