The University of Texas Health Science Center
2 ARTICLES PUBLISHED IN JoVE
Behavior
A Community-based Stress Management Program: Using Wearable Devices to Assess Whole Body Physiological Responses in Non-laboratory Settings
Robert Carter III 1, Kirtigandha Salwe Carter 2, John Holliday 3, Alice Holliday 3, Carlton Keith Harrison 4
1Department of Emergency Medicine, The University of Texas Health Science Center, 2Department of Integrative Physiology, The University of North Texas Health Science Center, 3Works of Wonder International, 4DeVos Graduate Sports Business Management Program, University of Central Florida
Stress is an unavoidable and persistent component of life and holistic approaches for its management are being considered. A standardized methodology was created to demonstrate the feasibility of a breath-based stress management protocol that can be used with commercially available portable technology.
Cancer Research
Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
Lindsey K. Sablatura 1, Kristin M. Bircsak 2, Peter Shepherd 3, Karla Queiroz 4, Mary C. Farach-Carson 1,5,6, Pamela E. Constantinou 1,7, Anthony Saleh 2, Nora Navone 3, Daniel A. Harrington 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center
This protocol demonstrates methods to enable extended in vitro culture of patient-derived xenografts (PDX). One step enhances overall viability of multicellular cluster cultures in 3D hydrogels, through straightforward removal of non-viable single cells. A secondary step demonstrates best practices for PDX culture in a perfused microfluidic platform.
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