University of Veterinary Medicine Hannover
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
Graham Brogden *1,2, Ariane Neumann *1,3, Diab M. Husein 1, Friederike Reuner 1,4, Hassan Y. Naim 1, Maren von Köckritz-Blickwede 1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.
Medicine
A Model to Simulate Clinically Relevant Hypoxia in Humans
Lars Eichhorn 1, Florian Kessler 1, Volker Böhnert 2, Felix Erdfelder 1, Anja Reckendorf 3, Rainer Meyer 4, Richard K. Ellerkmann 1
1Department of Anaesthesiology and Intensive Care Medicine, University Hospital of Bonn, 2Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, 3Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, 4Institute of Physiology 2, University of Bonn
Hypoxia simulation in humans has usually been performed by inhaling hypoxic gas mixtures. For this study, apneic divers were used to simulate dynamic hypoxia in humans. Additionally, physiological changes in desaturation and re-saturation kinetics were evaluated with non-invasive tools such as Near-Infrared-Spectroscopy (NIRS) and peripheral oxygenation saturation (SpO2).
