U.S. Army Medical Research Institute of Infectious Diseases

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines

Emily D. Cisney¹, Stefan Fernandez¹, Shannan I. Hall¹, Gale A. Krietz¹, Robert G. Ulrich¹

¹U.S. Army Medical Research Institute of Infectious Diseases

Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.