Rutgers - The State University of New Jersey

2 ARTICLES PUBLISHED IN JoVE

Biology

Analyzing and Building Nucleic Acid Structures with 3DNA

Andrew V. Colasanti¹, Xiang-Jun Lu², Wilma K. Olson¹

¹Department of Chemistry & Chemical Biology and BioMaPS Institute for Quantitative Biology, Rutgers - The State University of New Jersey, ²Department of Biological Sciences, Columbia University

The 3DNA software package is a popular and versatile bioinformatics tool with capabilities to analyze, construct, and visualize three-dimensional nucleic acid structures. This article presents detailed protocols for a subset of new and popular features available in 3DNA, applicable to both individual structures and ensembles of related structures.

Immunology and Infection

Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways

Paul J. Brennan¹, Nicolas W. S. Caballes¹, Anjanique Mercado-Feliciano¹, Yen-Fei Wu¹, Darin L. Wiesner¹

¹Department of Medicine, New Jersey Medical School, Rutgers - The State University of New Jersey

We present a method for a fungal pathogenesis model that preserves the natural positioning of fungal spores in the lung airways for analysis via fluorescent microscopy.