New York University Center for Health Informatics and Bioinformatics

1 ARTICLES PUBLISHED IN JoVE

Biology

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Julie Chaumeil¹, Mariann Micsinai^1,2,3,4, Jane A. Skok¹

¹Department of Pathology, New York University School of Medicine, ²New York University Center for Health Informatics and Bioinformatics, ³NYU Cancer Institute, ⁴Department of Pathology and Yale Cancer Center, Yale University School of Medicine

Here we describe a protocol for simultaneous detection of histone modifications by immunofluorescence and DNA sequences by DNA FISH followed by 3D microscopy and analyses (3D immuno-DNA FISH).