Inselspital, Bern University Hospital, University of Bern

8 ARTICLES PUBLISHED IN JoVE

Medicine

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Lukas Andereggen^3,4,5, Volker Neuschmelting^1,6, Michael von Gunten⁷, Hans Rudolf Widmer⁵, Jukka Takala¹, Stephan M. Jakob¹, Javier Fandino^1,2, Serge Marbacher^1,2

¹Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), ²Department of Neurosurgery, Kantonsspital Aarau, ³Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital, ⁴Harvard Medical School, Boston Children's Hospital, ⁵Department of Neurosurgery, University and Bern University Hospital (Inselspital), ⁶Department of Neurosurgery, University Hospital Cologne, ⁷Institute of Pathology, Länggasse Bern

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

Medicine

Discovering Middle Ear Anatomy by Transcanal Endoscopic Ear Surgery: A Dissection Manual

Lukas Anschuetz^1,2, Livio Presutti², Daniele Marchioni³, Marco Bonali², Wilhelm Wimmer^1,4, Domenico Villari², Marco Caversaccio^1,4

¹Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Modena, ³Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Verona, ⁴Artificial Hearing Research, Artorg Center for Biomedical Engineering, University of Bern

The aim of this article is to describe the methodology of exclusively endoscopic cadaveric middle ear dissection. Moreover, we aim to provide a comprehensive guide to endoscopic middle ear anatomy.

Neuroscience

Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Stefan Wanderer^1,2, Claudia Waltenspuel², Basil E. Grüter^1,2, Fabio Strange^1,2, Sivani Sivanrupan², Luca Remonda³, Hans Rudolf Widmer⁴, Daniela Casoni⁵, Lukas Andereggen^1,2, Javier Fandino^1,2, Serge Marbacher^1,2

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Department for Biomedical Research, Faculty of Medicine, University of Bern

Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

Medicine

The Microscopic Transcanal Approach in Stapes Surgery Revisited

Georgios Mantokoudis¹, Stefan Weder¹, Lukas Anschuetz¹, Sandra Gunser¹, Urs Borner¹, Marco D. Caversaccio¹

¹Department of Otorhinolaryngology, Head and Neck Surgery, lnselspital, Bern University Hospital, University of Bern

This article describes the microscopic transcanal technique for stapes surgery, providing step-by-step surgical instructions for familiarizing surgeons with this approach.

Medicine

Performing Intracochlear Electrocochleography During Cochlear Implantation

Klaus Schuerch^1,2, Manuel Waser¹, Georgios Mantokoudis¹, Lukas Anschuetz¹, Wilhelm Wimmer^1,2, Marco Caversaccio^1,2, Stefan Weder¹

Electrocochleography (ECochG) measures inner ear potentials generated in response to acoustic stimulation. In cochlear implant (CI) candidates, such inner ear potentials can be measured directly with the implant electrodes. In this video, we systematically explain how to perform ECochG recordings during CI surgery.

Medicine

Endoscopic Cholesteatoma Surgery

Sven Beckmann¹, Georgios Mantokoudis¹, Stefan Weder¹, Urs Borner¹, Marco Caversaccio¹, Lukas Anschuetz¹

¹Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern

The present protocol describes a step-by-step guide for the complete endoscopic removal of epitympanic cholesteatoma with different techniques for cholesteatoma dissection and bone removal for epitympanectomy.

Neuroscience

Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model

Stefan Wanderer^*1,2, Basil E. Grüter^*1,2, Jeannine Kümin^1,2, Gwendoline Boillat^1,2, Sivani Sivanrupan², Kristina Catalano^1,2, Michael von Gunten³, Hans Rudolf Widmer⁴, Serge Marbacher^1,2,5, Lukas Andereggen^1,2,5

¹Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Institute of Pathology Laenggasse, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Faculty of Medicine, University of Bern

We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.

Medicine

Robotic Cochlear Implantation for Direct Cochlear Access

Marco Caversaccio^1,2, Georgios Mantokoudis¹, Franca Wagner³, Philipp Aebischer^1,2, Stefan Weder^1,2, Wilhelm Wimmer^1,2

¹Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern, ³Department of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital

Robotic cochlear implantation is a procedure for minimally invasive inner ear access. Compared to conventional surgery, robotic cochlear implantation involves additional steps that need to be carried out in the operating room. In this article we give a description of the procedure and highlight the important aspects of robotic cochlear implantation.