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University of California at Los Angeles (UCLA)

3 ARTICLES PUBLISHED IN JoVE

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Biology

Dissection and Mounting of Drosophila Pupal Eye Discs
Joy S. Tea 1, Albert Cespedes 1, Daniel Dawson 2, Utpal Banerjee 1,3, Gerald B. Call 2
1Department of Molecular, Cell and Developmental Biology (MCDB), University of California at Los Angeles (UCLA), 2Arizona College of Osteopathic Medicine (AZCOM), Midwestern University, 3MCDB, Broad Stem Cell Research Center, UCLA

The goal of this technique is to enable researchers to perform dissection, immunostaining and mounting of pupal eye discs from Drosophila melanogaster of any age.

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Genetics

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
Gajendra W. Suryawanshi *1,2, Song Xu *3, Yiming Xie 1, Tom Chou 3, Namshin Kim 4, Irvin S. Y. Chen 1,5, Sanggu Kim 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)

This manuscript describes the experimental procedure and software analysis for a bidirectional integration site assay that can simultaneously analyze upstream and downstream vector-host junction DNA. Bidirectional PCR products can be used for any downstream sequencing platform. The resulting data are useful for a high-throughput, quantitative comparison of integrated DNA targets.

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Biochemistry

A Knowledge Graph Approach to Elucidate the Role of Organellar Pathways in Disease via Biomedical Reports
Alexander R. Pelletier 1,2,3, Dylan Steinecke 1,3,4, Dibakar Sigdel 1, Irsyad Adam 1, J. Harry Caufield 1, Vladimir Guevara-Gonzalez 1, Joseph Ramirez 1, Aarushi Verma 1, Kaitlyn Bali 1, Katherine Downs 1, Wei Wang 1,2,3, Alex Bui 3,4, Peipei Ping 1,2,3,4,5
1Department of Physiology, UCLA School of Medicine, 2Scalable Analytics Institute (ScAi) at Department of Computer Science, UCLA School of Engineering, 3NIH BRIDGE2AI Center at UCLA & NHLBI Integrated Cardiovascular Data Science Training Program, UCLA, 4Medical Informatics, University of California at Los Angeles (UCLA), 5Department of Medicine (Cardiology), UCLA School of Medicine

A computational protocol, CaseOLAP LIFT, and a use case are presented for investigating mitochondrial proteins and their associations with cardiovascular disease as described in biomedical reports. This protocol can be easily adapted to study user-selected cellular components and diseases.

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