Albert-Ludwigs-Universität Freiburg, Germany

1 ARTICLES PUBLISHED IN JoVE

Genetics

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Anja Greule¹, Songya Zhang¹, Thomas Paululat², Andreas Bechthold¹

¹Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-Universität Freiburg, Germany, ²Department of Chemistry and Biology, Universität Siegen

Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.