Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
Anush Arakelyan 1, Wendy Fitzgerald 1, Sonia Zicari 1, Murad Vagida 2, Jean-Charles Grivel 3, Leonid Margolis 1
1Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 2Laboratory of Atherothrombosis, Cardiology Department, Moscow State University of Medicine and Dentistry, 3Sidra Medical and Research Center
Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.
Immunology and Infection
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
Andrea Introini 1,2, Christophe Vanpouille 2, Wendy Fitzgerald 2, Kristina Broliden 1, Leonid Margolis 2
1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital, Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health
Infection of human tissues with human immunodeficiency virus (HIV) ex vivo provides a valuable 3D model of virus pathogenesis. Here, we describe a protocol to process and infect tissue specimens from human tonsils and female genital mucosae with HIV-1 and maintain them in culture at the liquid-air interface.
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