Hadassah - Hebrew University Medical Center
2 ARTICLES PUBLISHED IN JoVE
Genetics
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
Olivier Loudig 1,2,3, Christina Liu 1,2, Thomas Rohan 3, Iddo Z. Ben-Dov 4
1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center
Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.
Biology
A BW Reporter System for Studying Receptor-Ligand Interactions
Shlomo Elias 1,2, Shira Kahlon 2, Alexandra Duev-Cohen 2, Ofer Mandelboim 2
1Department of Hematology, Hadassah - Hebrew University Medical Center, 2The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC), Hadassah - Hebrew University Medical Center
This protocol describes how to establish a reporter system that can be used to identify and quantify receptor-ligand interactions.
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