Canadian Donation and Transplantation Research Program

2 ARTICLES PUBLISHED IN JoVE

Medicine

Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

Keir A. Forgie^1,2, Nicholas Fialka³, Mubashir Khan⁴, Max Buchko^1,2, Sanaz Hatami⁴, Sayed Himmat⁴, Xiao Qi⁴, Xiuhua Wang⁴, Katie-Marie Buswell⁵, Ryan Edgar⁵, Daina Domahidi⁵, Darren H. Freed^1,2,6,7, Jayan Nagendran^1,2,6,7

¹Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine, University of Alberta, ²Mazankowski Alberta Heart Institute, ³Faculty of Medicine and Dentistry, University of Alberta, ⁴Department of Surgery, Faculty of Medicine, University of Alberta, ⁵Ray Rajotte Surgical-Medical Research Institute (SMRI), University of Alberta, ⁶Alberta Transplant Institute, ⁷Canadian Donation and Transplantation Research Program

This protocol describes a juvenile porcine model of orthotopic left lung allotransplantation designed for use with ESLP research. Focus is made on anesthetic and surgical techniques, as well as critical steps and troubleshooting.

Medicine

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism

Keir A. Forgie^1,2, Nicholas Fialka³, Max Buchko^1,2, Sayed Himmat⁴, Sanaz Hatami⁴, Xiao Qi⁴, Xiuhua Wang⁴, Katie-Marie Buswell⁵, Ryan Edgar⁵, Darren H. Freed^1,2,6,7, Jayan Nagendran^1,2,6,7

This paper describes a porcine model of negative pressure ventilation ex situ lung perfusion, including procurement, attachment, and management on the custom-made platform. Focus is made on anesthetic and surgical techniques, as well as troubleshooting.