Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization MicroscopySrikant Ojha 1, Jan Pribyl 2, Simon Klimovic 2,3, Daniel Hadraba 4, Marketa Jirouskova 1, Martin Gregor 1
1Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU, Masaryk University, 3Department of Biochemistry, Faculty of Science, Masaryk University, 4Laboratory of Biomathematics, Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences
We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections.
