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Army Medical University (Third Military Medical University)

5 ARTICLES PUBLISHED IN JoVE

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Biology

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
Qianfan Bai *1, Zhiqiang Bai *1, Shaohai Xu 3, Lei Sun 1,2
1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University

An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.

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Cancer Research

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
Jenny Lazarus 1, Yagiz Akiska 1, Mirna Perusina Lanfranca 1, Lawrence Delrosario 1, Lei Sun 1, Daniel Long 2, Jiaqi Shi 3, Howard Crawford 2, Marina P. Di Magliano 1, Weiping Zou 1, Timothy Frankel 1
1Department of Surgery, University of Michigan, 2Department of Molecular and Cellular Physiology, University of Michigan, 3Department of Pathology, University of Michigan

Multiplex fluorescent immunohistochemistry is an emerging technology that enables the visualization of multiple cell types within intact formalin-fixed, paraffin embedded (FFPE) tissue. Presented are guidelines for ensuring a successful 7-color multiplex with instructions for optimizing antibodies and reagents, preparing slides, design and tips for avoiding common problems.

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Genetics

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
Kayli Sieber 1, Maya Saar 1, Comzit Opachaloemphan 2, Matthew Gallitto 3, Huan Yang 2, Hua Yan 1,4
1Department of Biology, University of Florida, 2Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 3Department of Radiation Oncology, Columbia University Medical Center, 4Center for Smell and Taste, University of Florida

Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

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Medicine

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Xi Ling 1, Peng Zou 1, Lin Ao 1, Niya Zhou 1, Xiaogang Wang 2, Lei Sun 1, Huan Yang 1, Jinyi Liu 1, Jia Cao 1, Qing Chen 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

The present protocol provides a practical solution that allows for the measurement of apoptosis, mitochondrial membrane potential, and DNA damage in human sperm with a single cytometer.

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Medicine

Transoral Robotic Total Thyroidectomy and Bilateral Central Regional Lymph Node Dissection for Papillary Thyroid Carcinoma
Shu Zhang 1, Gang Zhang 1, Jing Xu 1, Yan Jiang 1, Yan Xu 1
1Department of Breast and Thyroid Surgery, Daping Hospital, Army Medical University (Third Military Medical University)

The transoral robotic total thyroidectomy and bilateral central regional lymph node dissection using three ports without axillary incision are feasible and safe for early-stage papillary thyroid carcinoma. Here we present the operative techniques of this operation.

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