Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin

Fiorenza Orlando^*1, Chiara Paolini^*2, Silvia Agarbati², Cecilia Tonnini², Antonella Grieco², Chiara Capelli³, Martino Introna³, Mauro Provinciali¹, Armando Gabrielli², Gianluca Moroncini²

¹Servizio di Allevamento e Sperimentazione Animale, Polo ScientificoTecnologico, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)-Istituto Nazionale Ricovero e Cura Anziani (INRCA), ²Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, ³UOS Centro di Terapia Cellulare "G. Lanzani", Azienda Socio Sanitaria Territoriale (ASST) Papa Giovanni XXIII

Here we present an effective method to investigate the antifibrotic activity of intravenously infused human mesenchymal stromal cells obtained from the whole umbilical cord following the induction of lung injury by an endotracheal injection of bleomycin in C57BL/6 mice. This protocol can be easily extended to the preclinical testing of other therapeutics.