ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in CellsSevgi Irtegun 1, Yasmin M. Ramdzan 1, Terrence D. Mulhern 1, Danny M. Hatters 1
1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute
The biarsenical dyes FlAsH and ReAsH bind specifically to tetracysteine motifs in proteins and can selectively label proteins in live cells. Recently this labeling strategy has been used to develop sensors for different protein conformations or oligomeric states. We describe the labeling approach and methods to quantitatively analyze binding.
