Fondazione Bruno Kessler

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Peering at Brain Polysomes with Atomic Force Microscopy

Lorenzo Lunelli¹, Paola Bernabò², Alice Bolner², Valentina Vaghi¹, Marta Marchioretto², Gabriella Viero²

¹Laboratory of Biomolecular Sequence and Structure Analysis for Health, Fondazione Bruno Kessler, ²Institute of Biophysics, CNR Unit at Trento

While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.