The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue CocultureEva-Maria Materne *1, Ilka Maschmeyer *1,2, Alexandra K. Lorenz 1, Reyk Horland 1,2, Katharina M. S. Schimek 1, Mathias Busek 3, Frank Sonntag 3, Roland Lauster 1, Uwe Marx 1,2
1Medical Biotechnology, Technische Universität Berlin, 2TissUse GmbH, 3Fraunhofer IWS
Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.