Free University of Berlin

2 ARTICLES PUBLISHED IN JoVE

Biology

Operant Learning of Drosophila at the Torque Meter

¹Department of Neurobiology, Free University of Berlin

Measuring the yaw torque of tethered Drosophila with the torque meter allows the neuroscientist exquisite control of the stimulus situation of the experimental animal. Together with the unique genetic tools available in the fruit fly, this paradigm is used for a wide variety of neurobiological research.

Immunology and Infection

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

Diana Pauly¹, Pablo A. Chacana², Esteban G. Calzado³, Björn Brembs⁴, Rüdiger Schade⁵

¹Center for Biological Security, Robert Koch-Institute, ²CICVyA - INTA Castelar, Instituto de Virología, ³Center of Molecular Immunology, Ciudad de la Habana, Cuba, ⁴Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology, Free University of Berlin, ⁵Institut of Pharmacology, Charité-University Medicine of Berlin

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.