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Harvard T.H. Chan School of Public Health

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Rachel Daniels 1,2, Elizabeth J. Hamilton 2, Katelyn Durfee 2, Daouda Ndiaye 3, Dyann F. Wirth 2,5, Daniel L. Hartl 1, Sarah K. Volkman 2,4
1Department of Organismic and Evolutionary Biology, Harvard University, 2Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 3Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, 4School of Nursing and Health Sciences, Simmons College, 5Institute of Infectious Diseases, Broad Institute

While high resolution melting analysis offers the ability to differentiate between single nucleotide polymorphisms in a heterogeneous population, mutant allele amplification bias can increase its ability to detect alleles present at relatively low percentages within a sample. This protocol describes improvements that improve the sensitivity of high resolution melting analysis.

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Bioengineering

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
Hwanseok Jang 1, Jongseong Kim 1, Jennifer H. Shin 2, Jeffrey J. Fredberg 3, Chan Young Park 3, Yongdoo Park 1
1Department of Biomedical Sciences, Korea University, 2Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 3Department of Environmental Health, Harvard T.H. Chan School of Public Health

Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical gradient.

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Biology

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
Victoria A. Ingham 1, Andrew Bennett 2, Duo Peng 3, Simon C. Wagstaff 2, Hilary Ranson 1
1Vector Biology, Liverpool School of Tropical Medicine, 2Research Computing Unit, Liverpool School of Tropical Medicine, 3Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health

IR-TEx explores insecticide resistance-related transcriptional profiles in the species Anopheles gambiae. Provided here are full instructions for using the application, modifications for exploring multiple transcriptomic datasets, and using the framework to build an interactive database for collections of transcriptomic data from any organism, generated in any platform.

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Medicine

Integrated Spreadsheets for Nutritional Analysis of Population Diet Surveys
Sabri Bromage 1
1Department of Nutrition, Harvard T.H. Chan School of Public Health

This paper presents a system of integrated spreadsheets using simple formulas to calculate nutrient and food group intakes and the contributions of food groups to nutrient intakes for analysis of population diet survey data. The system accommodates quantitative, semiquantitative, and nonquantitative food intake data and a user-supplied food composition table.

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