Institute of Health Carlos III

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

María Ángeles Higueras¹, Lidia Jiménez-García¹, Sandra Herranz¹, Sonsoles Hortelano^*1, Alfonso Luque^*1

¹Pharmacological Therapies Unit, Research Institute for Rare Diseases, Institute of Health Carlos III

Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.