Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under MicrogravityRosangela Salerno-Goncalves 1, Alessio Fasano 2, Marcelo B. Sztein 1
1Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, 2Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children
Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.
