Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse ModelMatthew G. Wiet *1,2, Sayali Dharmadhikari *1,3, Audrey White 1,2, Susan D. Reynolds 4, Jed Johnson 5, Christopher K. Breuer 3,6, Tendy Chiang 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.
