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RIKEN Center for Integrative Medical Sciences

2 ARTICLES PUBLISHED IN JoVE

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Genetics

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Hazuki Takahashi *1, Harshita Sharma *1, Piero Carninci 1,2
1Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, 2TransSINE Technologies Co. Ltd.

SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.

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Neuroscience

Purification of the Dendritic Filopodia-rich Fraction
Yutaka Furutani 1,2, Yoshihiro Yoshihara 3
1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.

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