A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus SequencingLucas Stalder 1, Fabian Koechl 1, Kerstin Hahn 1, Marc Sultan 1, Megana K. Prasad 1
1Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Centre Basel
The study describes a simple, quick, and partially automated protocol to isolate high-quality nuclei from frozen mammalian tissues for downstream single nuclei RNA sequencing.
