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Sigma Life Science

3 ARTICLES PUBLISHED IN JoVE

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Biology

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Matthew J. Coussens *1, Kevin Forbes *1, Carol Kreader *1, Jack Sago 1, Carrie Cupp 1, John Swarthout 1
1Sigma-Aldrich

The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.

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Biology

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Keith Hansen 1, Matthew J. Coussens 1, Jack Sago 1, Shilpi Subramanian 1, Monika Gjoka 1, Dave Briner 1
1Emerging Technologies, Sigma Life Science

The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.

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Biology

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
Matthew J. Coussens 1, Courtney Corman 1, Ashley L. Fischer 1, Jack Sago 1, John Swarthout 1
1Sigma-Aldrich

Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.

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