Sinai Health System

2 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Tali Farine^1,2, Michael Parsons², Stephen Lye^1,2,3, Oksana Shynlova^1,2,3

¹Department of Physiology, University of Toronto, ²Lunenfeld-Tanenbaum Research Institute, Sinai Health System, ³Department of Obstetrics and Gynecology, University of Toronto

This protocol demonstrates a method for the isolation of primary human decidual cells collected from the fetal membranes of term placentae which can be used for a variety of applications (i.e. immunocytochemistry, flow cytometry, etc.) aiming to study the role of different cell populations in pregnancy complications.

Biochemistry

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Louis Masclef^*1, Uriarte Maxime^*1, Oumaima Ahmed¹, Nadine Sen Nkwe¹, Haithem Barbour¹, Nicholas V.G. Iannantuono¹, Amina Boubekeur¹, Salima Daou^1,2, El Bachir Affar^1,2

¹Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, ²Lunenfeld-Tanenbaum Research Institute, Sinai Health System

Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.