State University of New York Downstate Medical Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

Rafael E. Flores-Obando¹, Mona M. Freidin², Charles K. Abrams²

¹Program in Molecular and Cellular Biology, State University of New York Downstate Medical Center, ²Department of Neurology and Rehabilitation, University of Illinois at Chicago

We describe the immunomagnetic isolation of primary mouse oligodendrocytes, which allows the rapid and specific isolation of the cells for in vitro culture.

Bioengineering

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Seyed Mohammad Mir¹, Jiawen Chen¹, Meghan R. Pinezich¹, John D. O’Neill³, Brandon A. Guenthart⁴, Gordana Vunjak-Novakovic², Jinho Kim¹

¹Department of Biomedical Engineering, Stevens Institute of Technology, ²Department of Biomedical Engineering, Columbia University, ³Department of Cell Biology, State University of New York Downstate Medical Center, ⁴Department of Cardiothoracic Surgery, Stanford University

The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.