Methods to Classify Cytoplasmic Foci as Mammalian Stress GranulesAnaïs Aulas *1,2, Marta M. Fay *1,2, Witold Szaflarski 1,2,3, Nancy Kedersha 1,2, Paul Anderson 1,2, Pavel Ivanov 1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.
