JoVE Logo

Sign In

The Hussman Institute for Autism

1 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays
Andre W. Phillips 1, Jonathan E. Nestor 1, Michael W. Nestor 1
1The Hussman Institute for Autism

Here we report a protocol to develop a three-dimensional (3-D) system from human induced-pluripotent stem cells (hiPSCs) called the serum free embryoid body (SFEB). This 3-D model can be used like an organotypic slice culture to model human cortical development and for the physiological interrogation of developing neural circuits.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved