JoVE Logo

Sign In

The Nippon Dental University

1 ARTICLES PUBLISHED IN JoVE

image

Developmental Biology

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
Li Xiao 1, Hisashi Okamura 2, Yasuo Kumazawa 2
1Department of Pharmacology, The Nippon Dental University, 2Department of Oral and Maxillofacial Surgery, The Nippon Dental University Hospital

The goal of the protocol is to build an inflammatory human gingiva model in vitro. This tissue model co-cultivates three types of human cells, HaCaT keratinocytes, gingival fibroblasts, and THP-1 macrophages, under three-dimensional conditions. This model can be applied to investigating periodontal diseases, such as gingivitis and periodontitis.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved