Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic DiseaseNéstor Pallares-Lupon 1,2, Jason D. Bayer 1,2, Bastien Guillot 1,2, Guido Caluori 1,2, Girish S. Ramlugun 1,2, Kanchan Kulkarni 1,2, Virginie Loyer 1,2, Stephane Bloquet 1,2, Dounia El Hamrani 1,2, Jérôme Naulin 1,2, Marion Constantin 1,2, Pierre Dos Santos 1,2,3, Olivier Bernus 1,2, Pierre Jaïs 1,2,3, Philippe Pasdois 1,2, Richard D. Walton 1,2
1Centre de recherche Cardio-Thoracique de Bordeaux, Univ. Bordeaux, 2IHU Liryc, Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Univ., 3Electrophysiology and Ablation Unit, Bordeaux University Hospital (CHU)
Here, we present a protocol to obtain high resolution micro computed tomography images of healthy and pathological large mammalian whole hearts with collagen-selective contrast enhancement.
