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University of Minnesota - Twin Cities

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
Anthony J. Sprangers 1, Brian T. Freeman 1, Nicholas A. Kouris 1, Brenda M. Ogle 2
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Department of Biomedical Engineering, Materials Science Program, Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison

A method to track cell fusion in living organisms over time is described. The approach utilizes Cre-LoxP recombination to induce luciferase expression upon cell fusion. The luminescent signal generated can be detected in living organisms using biophotonic imaging systems with a sensitivity of detection of ˜1,000 cells in peripheral tissues.

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JoVE Journal

Ethylene Polymerizations Using Parallel Pressure Reactors and a Kinetic Analysis of Chain Transfer Polymerization
Ryan J. Hue 1, Ian A. Tonks 1
1Department of Chemistry, University of Minnesota - Twin Cities

A protocol for the high-throughput analysis of polymerization catalyst, chain transfer polymerizations, polyethylene characterization, and reaction kinetic analysis is presented.

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Cancer Research

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
Tanner J. McArdle 1, Brenda M. Ogle 1,2,3,4,5, Felicite K. Noubissi 6
1Department of Biomedical Engineering, University of Minnesota - Twin Cities, 2Stem Cell Institute, University of Minnesota - Twin Cities, 3Masonic Cancer Center, University of Minnesota - Twin Cities, 4Lillehei Heart Institute, University of Minnesota - Twin Cities, 5Institute for Engineering in Medicine, University of Minnesota - Twin Cities, 6Department of Biology, Jackson State University

This protocol describes an inverted vertical invasion assay that could be used to quantify the migration and invasion capabilities of cells in a three-dimensional setting while preserving the cell microenvironment. This assay is suitable for rare and/or sensitive cells.

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