Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing DiscRosario Vicidomini 1, Giuseppe Tortoriello 1, Maria Furia 1, Gianluca Polese 1
1Dipartimento di Biologia Strutturale e Funzionale, University of Naples
Laser microdissection was applied to analyse gene expression profiling in specific compartments of Drosophila wing disc subjected to localised RNAi in vivo. RNA extracted from equivalent areas of silenced and unsilenced compartments was analysed by quantitative RT-PCR to determine comparative gene expression profiling within the context of native tissue microecology.
