Preparation of Mouse Retinal Cryo-sections for ImmunohistochemistryHélène Léger 1, Evelyn Santana 2, William A Beltran 2, Francis C Luca 1
1Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, University of Pennsylvania School of Veterinary Medicine
This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.
