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University of Wisconsin - Milwaukee

4 ARTICLES PUBLISHED IN JoVE

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Biology

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
George M. Varsegi 1, Vinod Shidham 1
1Department of Pathology, University of Wisconsin - Milwaukee

Shidham's method for preparation of cell blocks with AV-marker from cytology specimens containing individually scattered cells and small cell groups.

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Biology

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies
Vinod B. Shidham 1, George M. Varsegi 1, Krista D'Amore 1, Anjani Shidham 2
1Department of Pathology, University of Wisconsin - Milwaukee, 2BioInnovation LLC

Practicing of fine needle aspiration biopsies (FNAB) by trainees is relatively challenging, due to the lack of an easily available, appropriate lesion. Preparation of an AV phantom lesion for practicing the FNAB procedure and mastering proficiency is relatively easy.

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Medicine

Performing and Processing FNA of Anterior Fat Pad for Amyloid
Vinod B. Shidham 1,2, Bryan Hunt 1, Safwan S. Jaradeh 3, Alexandru C. Barboi 3, Sumana Devata 4, Parameswaran Hari 5
1Department of Pathology, Medical College of Wisconsin, 2Current Address: Department of Pathology, Wayne State University School of Medicine Detroit Medical Center, 3Department of Neurology, Medical College of Wisconsin, 4Department of Medicine, Medical College of Wisconsin, 5Division of Neoplastic Diseases and Related Disorders, Medical College of Wisconsin

Fat pad aspiration is a preferred, minimally invasive, and low cost approach as compared to other methods to detect amyloid for diagnosis of systemic amyloidosis. This video article demonstrates a procedural outline for performing fat pad aspiration with appropriate processing of the specimen for the optimal diagnostic outcome.

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Biology

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
Michael Stoneman 1, Deo Singh 1, Valerica Raicu 1,2
1Department of Physics, University of Wisconsin - Milwaukee, 2Department of Biological Sciences, University of Wisconsin - Milwaukee

By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study.

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