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University of the Sciences

5 ARTICLES PUBLISHED IN JoVE

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Biology

Digital Microfluidics for Automated Proteomic Processing
Mais J. Jebrail 1, Vivienne N. Luk 1,2, Steve C. C. Shih 2,3, Ryan Fobel 2,3, Alphonsus H. C. Ng 2,3, Hao Yang 1, Sergio L. S. Freire 1, Aaron R. Wheeler 1,2,3
1Department of Chemistry, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto

Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.

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JoVE Core

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
Sergio L. S. Freire 1, Nathaniel Thorne 1, Michael Wutkowski 1, Selina Dao 1
1Department of Mathematics, Physics and Statistics, University of the Sciences

The protocol for fabrication and operation of field dewetting devices (Field-DW) is described, as well as the preliminary studies of the effects of electric fields on droplet contents.

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Environment

An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Trevor Cross 1, Courtney Schoff 2, Dylan Chudoff 3, LIbby Graves 3, Haley Broomell 4, Katrina Terry 4, Jennifer Farina 5, Alexandra Correa 5, David Shade 5, David Dunbar 5
1Biology Department, University of the Sciences, 2Biology Department, Arcadia University, 3Biology Department, Immaculata University, 4Biology/Clinical Laboratory Science, Neumann University, 5Science Department, Cabrini College

We present an enrichment protocol for the isolation of bacteriophages infecting bacteria in the Arthrobacter genus. This enrichment protocol produces fast and reproducible results for the isolation and amplification of Arthrobacter phages from soil isolates.

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Developmental Biology

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Gurpreet Kaur 1,2, Jaimo Ahn 1, Kurt D. Hankenson 1,3, Jason W. Ashley 1,4
1Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences, University of the Sciences, 3The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 4Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University

Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.

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Neuroscience

Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster
Kirby M. Donnelly 1, Margaret M. P. Pearce 1
1Department of Biological Sciences, University of the Sciences

Accumulating evidence supports the idea that pathogenic protein aggregates associated with neurodegenerative diseases spread between cells with prion-like properties. Here, we describe a method that enables visualization of cell-to-cell spreading of prion-like aggregates in the model organism, Drosophila melanogaster.

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