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VA Palo Alto Health Care System

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice
Devsmita Das 1,2, Cristy Phillips 3, Bill Lin 1, Fatemeh Mojabi 1, Mehmet Akif Baktir 2, Van Dang 1,2, Ravikumar Ponnusamy 1, Ahmad Salehi 1,2
1VA Palo Alto Health Care System, 2Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 3Department of Physical Therapy, Arkansas State University

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.

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