Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, SpotitonWilliam C. Budell 1, Luis Allegri 2, Venkat Dandey 1, Clinton S. Potter 1, Bridget Carragher 1
1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 2piTree Software
The protocol presented here describes the use of Spotiton, a novel robotic system, to deliver two samples of interest onto a self-wicking, nanowire grid that mix for a minimum of 90 ms prior to vitrification in liquid cryogen.
