biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

neuronal culture techniques

This collection features a variety of neuronal culture techniques, ranging from basic neuron cultures to advanced protocols for generating neural progenitors. It covers specific neuron cultures that employ three-dimensional scaffolds or microfluidics, and differentiation into brain organoids and networks. Techniques also encompass co-cultures to study neuron-glia interactions and synapse formation, among other approaches for exploring neuronal behavior and neurogenesis in vitro.

Neuronal Culture Techniques

neurophysiology

This collection presents methodologies for studying neural function and behavior. It features direct measurement techniques for recording electrical activities from neurons in diverse settings, and optical techniques for visualizing dynamic neuronal processes, tracking real-time changes, and examining spatial relationships within neural circuits. Additionally, it provides various stimulation methods using electrical, magnetic, and optogenetic tools to explore and modulate neural circuit responsiveness.

Neurophysiology

neuroimaging

This collection offers advanced methodologies for investigating brain structure, functional connectivity, and molecular dynamics in the nervous system. Focusing on in vitro and in vivo models, advanced imaging, and analytical techniques, these protocols equip researchers with innovative tools to study neural activity and pathologies across diverse areas.

Neuroimaging

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neuropathology

This collection offers advanced methodologies for investigating molecular mechanisms, structural changes, and functional disruptions in nervous system disorders. Focusing on in vitro and in vivo models, advanced imaging, and molecular analyses, these protocols equip researchers with innovative tools to study brain and neural pathologies across diverse areas.

Neuropathology

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neurotherapeutics

This collection presents advanced experimental methodologies for delivering therapeutic agents and modulating neural circuits in the central nervous system. Spanning gene therapy, neurostimulation, stem cell transplantation, and targeted drug delivery, these protocols empower researchers to explore and manipulate neurological functions and disease mechanisms in both in vivo and in vitro models.		

Neurotherapeutics

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primary neuronal culture

Primary Neuronal Culture

co-culture and interaction studies

Co-culture and Interaction Studies

advanced neuronal models

Advanced Neuronal Models

a technique to culture mouse hippocampal neurons

a-technique-to-culture-mouse-hippocampal-neurons

A Technique to Culture Mouse Hippocampal Neurons

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Preparing glia cell feeder dishes ...

generating a low-density primary neuron culture from frozen embryonic neurons

generating-low-density-primary-neuron-culture-from-frozen-embryonic

Generating a Low-Density Primary Neuron Culture From Frozen Embryonic Neurons

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Plate coating

	Coat a 96-well ...

culturing fluorescent excitatory neurons from a transgenic mouse pup brain

culturing-fluorescent-excitatory-neurons-from-transgenic-mouse-pup

Culturing Fluorescent Excitatory Neurons from a Transgenic Mouse Pup Brain

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Coating Glass Coverslips with ...

generating a primary culture of astrocytes from a rat pup brain

generating-a-primary-culture-of-astrocytes-from-a-rat-pup-brain

Generating a Primary Culture of Astrocytes from a Rat Pup Brain

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Primary cell culture preparation

...

isolation of microglia from the cortex of an adult mouse brain

isolation-of-microglia-from-the-cortex-of-an-adult-mouse-brain

Isolation of Microglia from the Cortex of an Adult Mouse Brain

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Mechanical Tissue Dissociation
...

a simple technique to isolate and culture murine astrocytes

a-simple-technique-to-isolate-and-culture-murine-astrocytes

A Simple Technique to Isolate and Culture Murine Astrocytes

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Rat or Mouse Brain Dissection
...

differentiating human induced pluripotent stem cells into neurons on micro-electrode arrays

differentiating-human-induced-pluripotent-stem-cells-into-neurons-on

Differentiating Human Induced Pluripotent Stem Cells into Neurons on Micro-Electrode Arrays

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Differentiation of ...

isolation and culture of primary neurons and glia from a rat urinary bladder

isolation-culture-primary-neurons-glia-from-rat-urinary

Isolation and Culture of Primary Neurons and Glia from a Rat Urinary Bladder

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

compartmentalized primary murine neuron culture using plastic microfluidic chips

compartmentalized-primary-murine-neuron-culture-using-plastic

Compartmentalized Primary Murine Neuron Culture Using Plastic Microfluidic Chips

isolation and culture of mouse primary cerebellar granule neurons

isolation-and-culture-of-mouse-primary-cerebellar-granule-neurons

Isolation and Culture of Mouse Primary Cerebellar Granule Neurons

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Experimental Preparation
NOTE: ...

culturing murine spiral ganglion neuron explants on multielectrode arrays

culturing-murine-spiral-ganglion-neuron-explants-on-multielectrode

Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Prepare Solutions for 
...

generation of primary retinal ganglion cell cultures from zebrafish embryos

generation-primary-retinal-ganglion-cell-cultures-from-zebrafish

Generation of Primary Retinal Ganglion Cell Cultures From Zebrafish Embryos

1. Preparation of solutions

	E2 media (1x)
	
		Prepare 100x E2A (500 mL), 500x E2B (100 mL) and 500x E2C (100 mL) solutions by combining all components ...

generation of neuronal cells from blood-derived pluripotent stem cells

generation-of-neuronal-cells-from-blood-derived-pluripotent-stem-cells

Generation of Neuronal Cells from Blood-Derived Pluripotent Stem Cells

Ethic approvals were obtained when performing the experiments.
1. Isolation of human peripheral blood mononuclear cells (PBMNCs)

	Ensure that all donors ...

isolation and culture of microglia from rat brains using serum-free media

isolation-culture-microglia-from-rat-brains-using-serum-free

Isolation and Culture of Microglia from Rat Brains using Serum-Free Media

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Prepare a Petri Dish for CD11b ...

isolation and culturing of adult neural stem cells from the mouse subcallosal zone

isolation-culturing-adult-neural-stem-cells-from-mouse-subcallosal

Isolation and Culturing of Adult Neural Stem Cells from the Mouse Subcallosal Zone

1. Preparation of Materials and Culture Medium

	For the dissection and dissociation of the SCZ (Subcallosal zone), wrap the brain matrix, double-edged ...

generating neural progenitors from mouse embryonic stem cells by the hanging drop method

generating-neural-progenitors-from-mouse-embryonic-stem-cells-hanging

Generating Neural Progenitors from Mouse Embryonic Stem Cells by the Hanging Drop Method

1. mESC culture

	Coat a 10 cm tissue-culture-treated plate with 0.1% gelatin and allow the gelatin to set for at least 15&#8211;30 min before aspirating ...

obtaining a mixed oligodendrocyte and astrocyte culture from adult mouse neural stem cells

obtaining-mixed-oligodendrocyte-astrocyte-culture-from-adult-mouse

Obtaining a Mixed Oligodendrocyte and Astrocyte Culture From Adult Mouse Neural Stem Cells

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Solutions and reagents

	Prepare ...

isolating induced neural progenitor cell colonies derived from adult human fibroblasts

isolating-induced-neural-progenitor-cell-colonies-derived-from-adult

Isolating Induced Neural Progenitor Cell Colonies Derived From Adult Human Fibroblasts

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Isolation of Putative Induced ...

generating an ultra-low-density neuronal culture using a high-density neuronal feeder layer

generating-an-ultra-low-density-neuronal-culture-using-high-density

Generating an Ultra-Low-Density Neuronal Culture Using a High-Density Neuronal Feeder Layer

1. 24-well Plates Preparation for High-Density Neuron Cultures
NOTE: Perform the following steps (2-3) the day before the planned harvest of embryos.

	...

differentiation of a human neuroblastoma cell line into mature neurons

differentiation-of-a-human-neuroblastoma-cell-line-into-mature-neurons

Differentiation of a Human Neuroblastoma Cell Line into Mature Neurons

1. General Considerations

	See the Table of Materials/Equipment for a list of necessary reagents. Perform all steps under strict aseptic conditions.
	Use ...

isolation of cells from sensory ganglia

Isolation of Cells from Sensory Ganglia

All procedures involving sample collection have been performed in accordance with the institute&#8217;s IRB guidelines.
1. Sensory Ganglia Dissociation

	...

dissociating and culturing neurons from hippocampal tissue samples

dissociating-and-culturing-neurons-from-hippocampal-tissue-samples

Dissociating and Culturing Neurons from Hippocampal Tissue Samples

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Dissection, Dissociation, and ...

preparation of colliculo-thalamocortical slices from a mouse pup brain

preparation-of-colliculo-thalamocortical-slices-from-a-mouse-pup-brain

Preparation of Colliculo-Thalamocortical Slices from a Mouse Pup Brain

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Preparation for and Removal of ...

culturing and maintaining dopaminergic neurons from mouse embryonic brain tissue

culturing-maintaining-dopaminergic-neurons-from-mouse-embryonic-brain

Culturing and Maintaining Dopaminergic Neurons from Mouse Embryonic Brain Tissue

a technique for harvesting and dissociating dorsal root ganglia for neuronal cultures

a-technique-for-harvesting-dissociating-dorsal-root-ganglia-for

A Technique for Harvesting and Dissociating Dorsal Root Ganglia for Neuronal Cultures

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Experimental Set-up

	Prior to ...

isolating and culturing murine cerebellar granular neuron progenitors

isolating-and-culturing-murine-cerebellar-granular-neuron-progenitors

Isolating and Culturing Murine Cerebellar Granular Neuron Progenitors

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1.&#160;Preparations

	Preparations ...

isolation of neural stem progenitor cells from the spinal cord periventricular region of an adult rat

isolation-neural-stem-progenitor-cells-from-spinal-cord

Isolation of Neural Stem Progenitor Cells from the Spinal Cord Periventricular Region of an Adult Rat

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee. 
1. Preparation of Dissection ...

isolating fluorescently labeled neurons from a murine brain

isolating-fluorescently-labeled-neurons-from-a-murine-brain

Isolating Fluorescently Labeled Neurons from a Murine Brain

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Manual Sorting of Fluorescently ...

preparation of collagen-coated compartmented culture dishes for neuronal cell culture

preparation-collagen-coated-compartmented-culture-dishes-for-neuronal

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture


	Compartmented culture of Rat Dorsal Root Ganglia (DRGs), Oligodendrocytes (OPCs), and hGCs (human glioma cells)

1. Preparation of compartmented culture ...

obtaining a single-cell suspension from mouse hippocampal tissue

obtaining-a-single-cell-suspension-from-mouse-hippocampal-tissue

Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee. 
1. Prepare Enzyme Mix 1 and 2 ...

preparing a single-cell suspension of immune cells from murine brain tissue

preparing-single-cell-suspension-immune-cells-from-murine-brain

Preparing a Single-Cell Suspension of Immune Cells from Murine Brain Tissue

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee. 
1. Preparing single-cell ...

isolating vestibular ganglion neurons from a mouse inner ear

isolating-vestibular-ganglion-neurons-from-a-mouse-inner-ear

Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Preparations
NOTE: The solutions ...

isolating murine primary oligodendrocytes using immunomagnetic beads

isolating-murine-primary-oligodendrocytes-using-immunomagnetic-beads

Isolating Murine Primary Oligodendrocytes Using Immunomagnetic Beads

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Mouse ...

isolating and culturing oligodendrocyte precursor cells from mouse pup brain cortex

isolating-culturing-oligodendrocyte-precursor-cells-from-mouse-pup

Isolating and Culturing Oligodendrocyte Precursor Cells from Mouse Pup Brain Cortex

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Tissue dissociation
NOTE: All the ...

isolation of schwann cells from mouse spinal roots

isolation-of-schwann-cells-from-mouse-spinal-roots

Isolation of Schwann Cells from Mouse Spinal Roots

generating dorsal root ganglion explant and dissociated cell culture

generating-dorsal-root-ganglion-explant-and-dissociated-cell-culture

Generating Dorsal Root Ganglion Explant and Dissociated Cell Culture

1. Explant culture of DRG from Adult Mouse

	Place the collected DRG in a 35 mm Petri dish containing 3 mL of ice-cold serum-free media (SFM).
	Transfer ...

obtaining horizontal mouse hippocampal brain slices

obtaining-horizontal-mouse-hippocampal-brain-slices

Obtaining Horizontal Mouse Hippocampal Brain Slices

 All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee. 
1. Preparation of ...

generation of tilted amygdala slices from a mouse brain

generation-of-tilted-amygdala-slices-from-a-mouse-brain

Generation of Tilted Amygdala Slices from a Mouse Brain

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Preparation of Acute Slices

	...

generating a coronal brain slice exposing the amygdala regions from a mouse brain

generating-coronal-brain-slice-exposing-amygdala-regions-from-mouse

Generating a Coronal Brain Slice Exposing the Amygdala Regions from a Mouse Brain

1. Preparation of Acute Slices

	Prepare ACSF, cutting solution, tools (scissors, scalpel, forceps, spatula, Pasteur pipette), and agar blocks.
	NOTE: 1 L ...

isolating microglia from mouse brain demyelinating lesions using magnetic activated cell sorting

isolating-microglia-from-mouse-brain-demyelinating-lesions-using

Isolating Microglia from Mouse Brain Demyelinating Lesions Using Magnetic Activated Cell Sorting

 All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee. 
1. Materials

	&#160;Prepare ...

isolation of multiple cell types from a mouse brain by mechanical homogenization

isolation-multiple-cell-types-from-mouse-brain-mechanical

Isolation of Multiple Cell Types from a Mouse Brain by Mechanical Homogenization

Disclaimer: &#160;All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
1. Mechanical ...

isolating immune cells from mouse choroid plexuses

isolating-immune-cells-from-mouse-choroid-plexuses

Isolating Immune Cells from Mouse Choroid Plexuses

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 
1. ...

Encyclopedia Of Experiments - neuroscience