A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

摘要

This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.

摘要

Microinjecting DNA into the cytoplasm of the syncytial gonad of Caenorhabditis elegans is the main technique used to establish transgenic lines that exhibit partial and variable transmission rates of extrachromosomal arrays to the next generation. In addition, transgenic animals are mosaic and express the transgene in a variable number of cells. Extrachromosomal arrays can be integrated into the C. elegans genome using UV irradiation to establish nonmosaic transgenic strains with 100% transmission rate of the transgene. To that extent, F1 progenies of UV irradiated transgenic animals are screened for animals carrying a heterozygous integration of the transgene, which leads to a 75% Mendelian transmission rate to the F2 progeny. One of the challenges of this method is to distinguish between the percentage of transgene transmission in a population before (X% transgenic animals) and after integration (≥75% transgenic F2 animals). Thus, this method requires choosing a nonintegrated transgenic line with a percentage of transgenic animals that is significantly lower than the Mendelian segregation of 75%. Consequently, nonintegrated transgenic lines with an extrachromosomal array transmission rate to the next generation ≤60% are usually preferred for integration, and transgene integration in highly transmitting strains is difficult. Here we show that the efficiency of extrachromosomal arrays integration into the genome is increased when using highly transmitting transgenic lines (≥80%). The described protocol allows for easy selection of several independent lines with homozygous transgene integration into the genome after UV irradiation of transgenic worms exhibiting a high rate of extrachromosomal array transmission. Furthermore, this method is quite fast and low material consuming. The possibility of rapidly generating different lines that express a particular integrated transgene is of great interest for studies focusing on gene expression pattern and regulation, protein localization, and overexpression, as well as for the development of subcellular markers.

引言

Transgenes are extensively used in C. elegans for a large range of applications. Transgenic strains are typically generated by DNA injection into the syncytial hermaphrodite gonad^1,2. The DNA carrying the transgene of interest is coinjected along with plasmids encoding coinjection markers^1,3,4. Coinjection markers are established transgenes leading either to the expression of a fluorescent protein or to a specific behavioral or morphological phenotype. The injected DNAs rearrange to form multi copy extrachromosomal arrays; thus the transgene of interest and the coinjection marker are transmitted together^5,4. Transgenic lines are selected in the F2 generation of injected animals by following the phenotype induced by the expression of the coinjection marker (fluorescence or specific phenotype)¹. Transgenic lines exhibit partial and variable transmission rates of the extrachromosomal arrays to the next generation. Depending on the strain, 10-100% (100% being a rare event) of the animals inherit extrachromosomal arrays. In addition, transgenic animals are mosaic and express the transgene in a variable number of cells. This mosaicism is likely to be correlated to the transmission rate to the next generation. Indeed, in both germ line and somatic cells the transmission rate depends on the segregation of the extrachromosomal arrays during cell divisions. One way to avoid this issue is to integrate the transgene into the genome. Classically, the integration of a transgene into a chromosome relies on irradiation (ultraviolet or gamma) of transgenic worms carrying extrachromosomal arrays³. Briefly, 50-100 hermaphrodite animals are irradiated at the L4 larval stage. In the F1 generation 200-800 transgenic animals are selected and individually cultured onto plates. According to Mendelian segregation, transgenic F1 animals carrying a heterozygous integration of the transgene into the genome would produce 1/2 of transgenic descendants that are heterozygous for the integrated array, 1/4 of transgenic descendants being homozygous for the integrated array, and 1/4 of descendants that have not integrated the array (Figure 1). Thus, plates containing the F2 generation are visually screened for ≥75% transgenic animals as observed by the expression of the coinjection marker. About 1/3 of the F2 animals selected for being integrant candidates are assumed to be homozygous for the integrated array and to produce 100% of homozygous F3 animals. Hence, from each selected plate, three to eight transgenic F2 animals are individually cultured and plates with 100% of transgenic descendants are selected. Next, eight F3 animals are individually cultured to confirm the 100% inheritance of the transgene.

The main disadvantages of this method are that

1. it requires a visual screen of several hundred F1 plates for ≥75% transgenic F2 progeny, which is time consuming due to the variable and unpredictable percentage of transmission of nonintegrated extrachromosomal arrays. Alternatively, to avoid the screening of F1 plates several subsequent starvation steps can be performed after irradiation of P0 animals; followed by picking 100 transgenic animals and screening for 100% transgenic progeny³.
2. it is not adapted for strains exhibiting a percentage of extrachromosomal array transmission higher than 60% as this percentage is difficult to distinguish this percentage from 75%.
3. as only strains with a relatively low transmission rate can be used, a high number of irradiated transgenic P0 animals is necessary as well as the observation of numerous F1 and F2 animals, which is highly time consuming and requires a considerable number of plates.

The irradiation of worms presumably induces double-strand breaks in the DNA and integration of extrachromosomal arrays into chromosomes occurs during DNA repair. Thus, it is likely that chances to obtain successful irradiation mediated integration are directly proportional to the number of germ line cells carrying an extrachromosomal array. Strains with a high transmission rate may contain more transgenic DNA arrays than strains with a low transmission rate as suggested in², and exhibit a higher number of germ line cells carrying extrachromosomal arrays. Thus, we reasoned that in highly transmitting lines, transgenes might be easier to integrate than in lines with a low transmission rate. However, the standard protocol excludes the possibility of integrating transgenes with high transmission frequency as the percentage of transgenic animals with a nonintegrated extrachromosomal array can hardly be distinguished from that of the progeny of a F1 animal carrying a heterozygous integration of the transgene into the genome (Figure 1).

Here we show that for a particular transgene, the number of integrated lines recovered after irradiation with ultraviolet (UV) light is dependent on the initial percentage of transmission of the nonintegrated array. We present an improved protocol for UV irradiation-mediated transgene integration that is particularly relevant to lines exhibiting a high rate of transgene array transmission.

研究方案

1. Culturing Transgenic Nematodes Prior to Integration

Transgenic lines can be generated as described in¹. These lines usually contain a coinjection marker plasmid leading either to the expression of a fluorescent protein or to a specific behavioral or morphological phenotype at a given development stage^1,3. This integration protocol can be applied to strains expressing fluorescent or nonfluorescent coinjection markers. In the example to follow, a strain with a fluorescent coinjection marker was used.

1. Prepare culture plates:
   1. Prepare about 500 60 mm large Petri dishes poured aseptically with 10 ml each of Nematode Growth Medium (NGM)^6,7.
   2. Seed each plate with 0.3 ml of saturated Escherichia coli OP50 culture following standard procedure^6,7.
2. Evaluate the transmission rate of the transgenic line to be integrated:
   1. For each transgenic line, pick ten fluorescent gravid adults onto ten separate culture plates under a fluorescent stereoscope using a worm pick as described in⁷.
   2. Culture the animals in a worm incubator at 15-25 °C (depending on the genetic background) to allow the worms to reproduce.
   3. Observe progeny each day to evaluate at which developmental stage the coinjection marker is expressed and can be best observed. The expression of most of the currently used coinjection markers is observed at the L4 larval stage or at adulthood.
   4. Evaluate the transmission rate of the progeny by determining the percentage of fluorescent progeny by observation under a fluorescent stereoscope.
3. Choose a transgenic line for transgene integration:
   1. Select the transgenic line with the highest transmission rate as possible (ideally ≥80%).
4. Obtain a population of transgenic animals synchronized at the L4 larval stage for integration:
   1. Pick 30 fluorescent gravid adults onto five culture plates (six animals by plate).
   2. Let the worms lay eggs for 3-4 hr at 15 °C.
   3. Eliminate the adults from the plates after checking for the presence of at least 30 eggs by plate.
   4. Culture the animals in a worm incubator at 15-25 °C until the progeny reaches the L4 larval stage (96 hr at 15 °C or 36 hr at 25 °C).

2. UV Irradiation and Recovery of Transgenic Worms

1. Worm irradiation:
   1. Pick 30-100 fluorescent transgenic L4 animals onto separate culture plates (15-20 animals by plate).
   2. Place the plates, with the lids removed, in a UV cross-linker. Irradiate the worms at 0.012 J/cm². Note that strains are more or less sensitive to UV and that results can be variable depending on the UV irradiator used. A dose range between 0.010-0.015 J/cm² can be tested.
2. Worm recovery after irradiation:
   1. Place plates with irradiated worms overnight at 15 °C for recovery.
   2. Check for the number animals that are alive. In our hands, a survival rate of around 80-90% is adapted for an efficient irradiation.
   3. Grow the irradiated animals at 15-25 °C until the progeny has reached the developmental stage allowing for the observation of the coinjection marker expression, as established in step 1.2.

3. Isolation of Integrated Transgenic Lines

1. Selection of F1 animals:
   1. Single 150-200 fluorescent F1 animals onto separate culture plates. For highly transmitting lines (≥80%), 100 F1 animals are enough.
   2. Keep these F1 plates at 15-25 °C until the progeny reaches an appropriate stage for the observation of fluorescent F2 animals.
   3. Discard all F1 plates exhibiting either i) no progeny, indicating that the F1 animal was sterile or died, or ii) no or only few fluorescent F2 animals, indicating that the F1 animal did not transmit the transgene at the expected rate. This step is quick and represents a real advantage when compared to the standard protocol that requires an estimation of the percentage of fluorescent F2 worms in order to select F1 plates exhibiting ≥75% fluorescent progeny (Table 1).
2. Selection of F2 animals:
   1. Single four fluorescent transgenic F2 animals from each selected F1 plate. When using fluorescent transgenes (for example mCherry⁸, gfp⁹, or dsRed¹⁰), pick F2 worms with a high level of fluorescence as this could indicate that these animals are homozygous for the integrated array. Note that when picking F2 animals it is critical not to carry along eggs or larvae, in order to avoid false negatives in the next step.
   2. Grow F2 animals at 15-25 °C.
3. Isolation and validation of integrated transgenic strains:
   1. Screen F2 plates for 100% fluorescent transgenic F3 worms. The screen is quite rapid as the presence of a single nonfluorescent worm indicates that the plate should be thrown away. A F2 animal homozygous for the integrated transgene will give 100% homozygous fluorescent progeny.
   2. Single eight fluorescent F3 animals from selected plates to confirm the 100% inheritance of the transgene. In theory, picking one animal is enough at this step. However, picking eight fluorescent F3 animals may be necessary to avoid selecting unfertile or unhealthy animals as irradiation can randomly induce mutations that affect genes important for worm physiology.
   3. If possible keep several independent integrated transgenic strains, i.e. strains recovered from different F1 animals.

结果

Integrations of transgenes in two different lines transmitting extrachromosomal arrays at about the same frequency (50-60%) were performed using the standard and the improved protocols (Table 1). The standard protocol allowed the recovery of three independent integrated lines after UV irradiation of 91 transgenic L4 animals, while the improved protocol allowed the recovery of one integrated line after UV irradiation of 30 transgenic L4 animals. This suggests that the efficiency of integration is similar ...

讨论

The protocol described here provides an efficient method to integrate extrachromosomal transgenic arrays into the genome of C. elegans. Using different nonintegrated lines, which transmitted the same transgene Pdyc-1::dyc-1::gfp (pKG45) at different rates (10-15%, 50-60%, and >80%), we showed that the number of integrated strains recovered after UV irradiation is dependent of the initial level of transmission of the nonintegrated line (Table 2). Therefore, transgenes are easier to i...

材料

Name	Company	Catalog Number	Comments
Agar	Prolabo	20768.292	for NGM (nematode growth medium) preparation (6,7)
NaCl	Chimie-Plus	40045	for NGM preparation
Bacto-peptone	BD	211820	for NGM preparation
KH2PO4	Roth	3904-1	for NGM preparation
K2HPO4	Prolabo	26930.293	for NGM preparation
MgSO4	Roth	P027.2	for NGM preparation
CaCl2	Prolabo	22317.297	for NGM preparation
Cholesterol	Rectapur		for NGM preparation
LB-broth	Sigma	L3022	for culture of OP50 bacteria
EQUIPMENT
U.V. cross-linker	Fischer Scientific		Wavelength 254 nm
Microscope SteREO Discovery V8	Carl Zeiss, Inc.
Petri dishes, 60 mm	CML	BPS55E6
Platinium wire 0.25 mm	Alfa Aesar	7440-06-4	To make a pick for worms (7)