electroencephalographic (eeg) electrode implantation on mouse brain

用低成本的 EEG 和毫米级线圈刺激小鼠大脑

Transcranial magnetic stimulation (TMS) is a promising tool for human brain science, clinical application, and animal model research because of its non-/low invasiveness. During the early stage of TMS applications, measurement of the cortical effect in response to single- and paired-pulse TMS in humans and animals was restricted to the motor cortex; easily measurable output was limited to motor evoked potentials and induced myoelectric potentials involving the motor cortex1,2. To expand the brain regions that can be measured by TMS modulation, electroencephalographic (EEG) recording was integrated with single- and paired-pulse TMS as a useful method to directly examine the excitability, connectivity, and spatiotemporal dynamics of areas throughout the whole brain3,4,5. Thus, the simultaneous application of TMS and EEG recording (TMS-EEG) to the brain has been used to probe various superficial cortical brain areas of humans and animals to investigate intracortical neural circuits (see Tremblay et al.6). Moreover, TMS-EEG systems can be used to examine additional cortical spatiotemporal characteristics, including the propagation of signals to other cortical areas and the generation of oscillatory activity7,8.
However, the mechanism of action of TMS in the brain remains speculative because of the non-invasiveness of TMS, which limits our knowledge of how the brain functions during TMS applications. Therefore, invasive translational studies in animals ranging from rodents to humans are of crucial importance to understand the mechanism of the effects of TMS on neural circuits and their activity. In particular, for combined TMS-EEG experiments in animals, a simultaneous stimulation and measurement system has not been intensively developed for small animals. Therefore, experimentalists are required to construct such a system by trial and error according to their specific experimental requirements. In addition, mouse models are useful among other in vivo animal species models because many transgenic and strain-isolated mice strains are available as biological resources. Thus, a convenient method to build a TMS-EEG-combined measurement system for mice would be desirable for many neuroscience researchers.
This study proposes a TMS-EEG-combined method that can be applied for simultaneous stimulation and recording of the mouse brain, which is the main type of transgenic animal used in research, and that can easily be constructed in typical neuroscience laboratories. First, a low-cost EEG recording system is described using conventional screw electrodes and a flexible substrate to reproducibly assign an electrode-array position in each experiment. Second, a magnetic stimulation system is constructed using a millimeter-sized coil, which can easily be custom-made in typical laboratories. Third, the TMS-EEG-combined system records neural activity in response to sound and magnetic stimulation. The method presented in this study can reveal the mechanisms that generate specific disorders in small animals, and the results obtained in the animal models can be translated to understand the corresponding human disorders.

作者聚焦： 低成本脑电图记录系统结合毫米级线圈经颅刺激小鼠体内大脑  

低成本脑电图记录系统结合毫米级线圈，在体内经颅刺激小鼠大脑

In neuroscience, TMS and EEG recording are frequently used to advance our understanding of the brain. TMS is especially promising due to its non-invasiveness. Combining TMS with EEG recording enables researchers to explore excitability, connectivity, and spatiotemporal dynamics across various brain regions.This protocol aims to develop a simultaneous stimulation measurement system for TMS-EEG experiments with small animals. These system have not been extensively developed, forcing researchers to create a system to trial and error according to the unique experimental requirements. This easily adaptable approach can be set up in standard neuroscience labs, making it more accessible and practical for researchers.The findings of this study will advance research and elucidating mechanisms behind specific disorders in small animals, which are essential models for human candidates. Further, these models will improve their understanding of human conditions and help guide the creation of more effective treatments and interventions.

Watch this Scientific Journal Video about Electroencephalographic EEG Electrode Implantation on Mouse Brain at JoVE.com

Electroencephalographic (EEG) Electrode Implantation on Mouse Brain  

脑电图（EEG）电极植入小鼠大脑

首先，使用腹膜内麻醉小鼠并使用标准外科手术暴露颅骨。将安装在柔性基板背面的 2D 电极阵列上的不锈钢棒连接到机械手。然后将柔性基材放在暴露的头骨上。调整阵列上通道 3 和 14 的位置，以适合下丘。用永久性标记在头骨上的通道 3、8、9 和 14 的位置绘制四个小圆圈，作为目标地标。接下来，干燥颅骨表面以提高牙科水泥的粘附性，并将柔性基板上的2D电极阵列与小鼠头骨电隔离。在颅骨表面涂上一层约1毫米厚的牙科水泥，让水泥固化约30分钟。将柔性基材与颅骨表面上的小圆形标记对齐。然后将牙钻的尖端放在柔性基板上的每个电极垫孔上，并小心地钻入颅骨。使用螺丝刀将微型螺旋电极固定在头骨的钻孔中。牢固地压接螺旋电极头和电极垫。最后，使用测试设备（例如LCR计）测量每个螺旋电极和连接器之间的电导率，以确认电导率。

electroencephalographic-eeg-electrode-implantation-on-mouse-brain

Microsoft Bing

Research

JoVE Journal

Neuroscience

Electroencephalographic (EEG) Electrode Implantation on Mouse Brain

1.0K 次观看.  Hokkaido University. 首先，使用腹膜内麻醉小鼠并使用标准外科手术暴露颅骨。将安装在柔性基板背面的 2D 电极阵列上的不锈钢棒连接到机械手。然后将柔性基材放在暴露的头骨上。调整阵列上通道 3 和 14 的位置，以适合下丘。用永久性标记在头骨上的通道 3、8、9 和 14 的位置绘制四个小圆圈，作为目标地标。接下来，干燥颅骨表面以提高牙科水泥的粘附性，并将柔性基板上的2D电极阵列...

视频: Electroencephalographic (EEG) Electrode Implantation on Mouse Brain  

Low-Cost Electroencephalographic Recording System Combined with a Millimeter-Sized Coil to Transcranially Stimulate the Mouse Brain In Vivo

A low-cost electroencephalographic (EEG) recording system is proposed here to drive transcranial magnetic stimulation (TMS) of the mouse brain in vivo, utilizing a millimeter-sized coil. Using conventional screw electrodes combined with a custom-made, flexible, multielectrode array substrate, multi-site recording can be carried out from the mouse brain. In addition, we explain how a millimeter-sized coil is produced using low-cost equipment usually found in laboratories. Practical procedures for fabricating the flexible multielectrode array substrate and the surgical implantation technique for screw electrodes are also presented, which are necessary to produce low-noise EEG signals. Although the methodology is useful for recording from the brain of any small animal, the present report focuses on electrode implementation in an anesthetized mouse skull. Furthermore, this method can be easily extended to an awake small animal that is connected with tethered cables via a common adapter and fixed with a TMS device to the head during recording.The present version of the EEG-TMS system, which can include a maximum of 32 EEG channels (a device with 16 channels is presented as an example with fewer channels) and one TMS channel device, is described. Additionally, typical results obtained by the application of the EEG-TMS system to anesthetized mice are briefly reported.

A low-cost electroencephalographic recording system combined with a millimeter-sized coil is proposed to drive transcranial magnetic stimulation of the mouse brain in vivo. Using conventional screw electrodes with a custom-made, flexible, multielectrode array substrate, multi-site recording can be carried out from the mouse brain in response to transcranial magnetic stimulation.

A low-cost electroencephalographic (EEG) recording system is proposed here to drive transcranial magnetic stimulation (TMS) of the mouse brain in vivo, ...

科研

神经科学

To begin, use an intraperitoneal anesthetized mouse and expose the skull Using standard surgical procedures. Attach the stainless steel rod mounted to the 2D electrode array on the backside of the flexible substrate to a manipulator. Then place the flexible substrate on the exposed skull.Adjust the location of channels 3 and 14 on the array to fit within the inferior colliculus. Draw four small circles at the locations of channels 3, 8, 9, and 14 on the skull with a permanent marker to serve as targeting landmarks. Next, dry the skull surface to improve the adherence of the dental cement and to electrically isolate the 2D electrode array on the flexible substrate from the mouse skull.Apply a layer of dental cement to the skull surface approximately one millimeter thick and allow the cement to cure for approximately 30 minutes. Align the flexible substrate in line with the small circular marks on the surface of the skull. Then position the tip of a dental drill on each electrode pad hole on the flexible substrate and carefully drill into the skull.Using a screwdriver, secure the miniature screw electrodes into the drill holes of the skull. Crimp the head of the screw electrode and the electrode pad firmly. Finally, measure the conductance between each screw electrode and the connector with testing equipment, such as an LCR meter to confirm electrical conductivity.